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Expression of RUNX2 and Osterix in Rat Mesenchymal Stem Cells during Culturing in Osteogenic-Conditioned Medium

  • Translated from Kletochnye Tekhnologii v Biologii i Meditsine (Cell Technologies in Biology and Medicine)
  • Published:
Bulletin of Experimental Biology and Medicine Aims and scope

We studied the expression of transcription factors RUNX2 and Osterix after addition of a concentrate of osteogenic-conditioned medium to the culture medium for osteogenic differentiation of mesenchymal stem cells (MSC). The obtained concentrate of osteogenic-conditioned medium containing a complex of bioactive substances with a molecular weight >10 kDa provided MSC differentiation into osteoblasts, which was confirmed by high level of expression of transcription factors RUNX2 and Osterix in comparison with the negative control. The highest expression of transcription factor Osterix was revealed on day 14 of MSC culturing in the presence of osteogenic supplement StemPro (positive control) and the studied concentrate of osteogenic-conditioned medium.

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Authors and Affiliations

  1. National Research Tomsk State University, Tomsk, Russia

    L. A. Pokrovskaya

  2. Research Laboratory “Cellular, Assisted Reproductive, and DNA Technologies”, Belgorod State National Research University, Belgorod, Russia

    S. V. Nadezhdin, E. V. Zubareva & E. S. Gnezdyukova

  3. Innovation Centre “Biruch — New Technologies”, Alekseevka, Belgorod region, Russia

    Yu. E. Burda

Authors
  1. L. A. Pokrovskaya
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  2. S. V. Nadezhdin
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  3. E. V. Zubareva
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  4. Yu. E. Burda
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  5. E. S. Gnezdyukova
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Corresponding author

Correspondence to S. V. Nadezhdin.

Additional information

Translated from Kletochnye Tekhnologii v Biologii i Meditsine, No. 2, pp. 112-117, June, 2020

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Pokrovskaya, L.A., Nadezhdin, S.V., Zubareva, E.V. et al. Expression of RUNX2 and Osterix in Rat Mesenchymal Stem Cells during Culturing in Osteogenic-Conditioned Medium. Bull Exp Biol Med 169, 571–575 (2020). https://doi.org/10.1007/s10517-020-04931-5

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  • DOI: https://doi.org/10.1007/s10517-020-04931-5

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