Taraxacum pieninicum Pawł. is listed as critically endangered species, for which currently applied protection methods are insufficient. The aim of this study was to investigate the possibility of T. pieninicum storage in the form of synthetic seeds under slow-growth conditions in combination with ABA treatment, as one of the ex situ protection methods of this species. The obtained results indicated that darkness was much more favorable condition for synseed storage and did not generate additional stress during cold exposure in contrast to the light conditions. The preculture of shoot tips on the medium supplemented with ABA led to a decrease in the shoots proliferation rate and inhibition of their growth. ABA clearly inhibited growth of the encapsulated shoot tips also during cold storage. Biochemical parameters showed that ABA effectively reduced the negative effect of the cold stress, what was found on the basis of analyzes of H₂O₂ and TBARS levels in the stored material. Moreover, synseeds stored under light conditions and treated with ABA exhibited decreased level of endogenous jasmonic acid what indicated interaction between those two phytohormones at a low temperature. The study also demonstrated that in vitro culture, cold storage and ABA treatment had no effect on the flowering process of this species after acclimatization to ex vitro conditions.

Taraxacum pieninicumPawł。被列为极度濒危物种，目前应用的保护方法不足。这项研究的目的是调查T. pieninicum的可能性在慢速生长条件下与ABA处理结合以合成种子的形式储存，作为该物种的非原生境保护方法之一。所获得的结果表明，与光条件相比，黑暗是用于合酶储存的更有利条件，并且在冷暴露期间不产生额外的应力。在添加了ABA的培养基上对茎尖进行预培养会导致茎尖增殖速率降低并抑制其生长。ABA在冷藏过程中也明显抑制了包封的芽尖的生长。根据对H ₂ O ₂的分析发现，生化参数表明ABA有效降低了冷胁迫的负面影响。和TBARS水平在存储的材料。此外，在光照条件下储存并用ABA处理过的合成物显示出降低的内源茉莉酸水平，这表明这两种植物激素在低温下相互作用。该研究还表明，在适应体外条件后，体外培养，冷藏和ABA处理对该物种的开花过程没有影响。