The aim of this work is to investigate the effect of the ethyl carbamate (EC) content in musalais on the metabolism of rats. Electron beam irradiation was performed to decrease the content of EC in musalais, and Sprague Dawley rats were subjected to intragastric administration of musalais with varying EC content (high, medium, and low groups). Control rats were fed normally without any treatment. Serum and urine samples were analyzed using ultra-high-performance liquid chromatography quadrupole time-of-flight mass spectrometry. Principal component analysis and orthogonal projections to latent structures discriminant analysis (OPLS-DA) were performed to detect changes in the metabolite profile in the serum and urine in order to identify the differential metabolites and metabolic pathways. The results demonstrated clear differences in the serum and urine metabolic patterns between control and treatment groups. Ions in treatment groups with variable importance in the projection of >1 (selected from the OPLS-DA loading plots) and Ps < 0.05 (Student t test) compared to control group were identified as candidate metabolites. Analysis of the metabolic pathways relevant to the identified differential metabolites revealed that high EC content in musalais (10 mg/kg) mainly affected rats through valine, leucine, and isoleucine biosynthesis and nicotinate and nicotinamide metabolism, which were associated with energy metabolism. In addition, this work suggests that EC can induce oxidative stress via inhibition of glycine content.

这项工作的目的是调查在穆沙拉氏中氨基甲酸乙酯（EC）含量对大鼠代谢的影响。进行电子束辐照以减少斑马中的EC含量，并对Sprague Dawley大鼠进行胃内施用具有不同EC含量的斑马（高，中和低组）。对照大鼠正常喂养而没有任何治疗。使用超高效液相色谱四极杆飞行时间质谱分析血清和尿液样品。进行主成分分析和对潜在结构判别分析的正交投影（OPLS-DA），以检测血清和尿液中代谢物谱的变化，以鉴定差异代谢物和代谢途径。结果表明，对照组和治疗组之间的血清和尿液代谢模式存在明显差异。处理组中的离子在> 1的投影中具有不同的重要性（从OPLS-DA加载图中选择），并且与对照组相比，P s <0.05（Student t检验）被确定为候选代谢产物。对与鉴定出的差异代谢物相关的代谢途径的分析表明，穆萨拉斯中的高EC含量（10 mg / kg）主要通过缬氨酸，亮氨酸和异亮氨酸的生物合成以及烟酸和烟酰胺代谢影响大鼠，这与能量代谢有关。此外，这项工作表明EC可以通过抑制甘氨酸含量来诱导氧化应激。