Abstract

In this study, 10 mg L^–1 L-tryptophan effects on the seedling growth (fresh weight, radicle length and radicle number), seed germination, chromosomal aberrations and mitotic activity in Allium cepa L. germinated in salinity (0.175 M NaCl) conditions were investigated. In only L-tryptophan medium, the radicle length and radicle number of the seedlings were partially reduced compared to the control seedlings grown in the pure water medium, the germination percentage and fresh weight indicate statistically the same values as the control. Moreover, the chromosomal aberrations and mitotic index in the root tip meristem cells of Allium cepa seeds germinated in alone L-tryptophan medium showed increase compared to the control seeds germinated in the pure water medium. On the other hand, salt stress significantly inhibited the germination rate, radicle length, radicle number and fresh weight of Allium cepa L. In addition, it important reduced the mitotic index (activity) and increased the chromosomal aberrations in root tip meristems of the seeds. Nevertheless, the inhibitory effects of salt stress on all the mentioned parameters were dramatically alleviated by L-tryptophan application. The germination percentage, radicle length, radicle number, fresh weight, mitotic index and chromosomal aberrations of the seedlings grown in 0.175 M salinity were 23%, 10.3 mm, 12.7, 7 g, 1.2 and 17% respectively, while these values became 77%, 15.3 mm, 16.7, 15.2 g, 11.6 and 14.6% in the seedlings treated with 10 mg L^–1 L-tryptophan.

中文翻译：

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盐胁迫下洋葱L-色氨酸的细胞遗传和生理效应。

摘要

在这项研究中，盐度（0.175 M NaCl）下发芽的洋葱葱（Allium cepa L. ）中10 mg L – ¹ L色氨酸对幼苗生长（鲜重，胚根长度和胚根数），种子萌发，染色体畸变和有丝分裂活性产生影响。被调查了。仅在L-色氨酸培养基中，与在纯水培养基中生长的对照幼苗相比，幼苗的胚根长度和胚根数目被部分降低，发芽百分数和鲜重在统计学上指示与对照相同。此外，葱属根尖分生组织细胞的染色体畸变和有丝分裂指数与在纯水培养基中发芽的对照种子相比，在单独的L-色氨酸培养基中发芽的种子显示出增加。另一方面，盐胁迫显着抑制洋葱的发芽率，胚根长度，胚根数和鲜重。L.此外，重要的是降低了种子的根尖分生组织中的有丝分裂指数（活性）并增加了染色体畸变。然而，L-色氨酸的施用显着减轻了盐胁迫对所有上述参数的抑制作用。在0.175M盐度下生长的幼苗的发芽率，胚根长度，胚根数，鲜重，有丝分裂指数和染色体畸变分别为23％，10.3mm，12.7、7g，1.2和17％，而这些值变为77％ 10 mg L ^–1 L-色氨酸处理的幼苗中，分别为15.3 mm，16.7、15.2 g，11.6和14.6％。