Introduction

Prostatitis is likely to occur in younger or middle-aged men, while prostate cancer is likely to occur in older men. Although amino acids and lipids as biomarkers of prostate cancer have been examined using prostate cancer cell lines/tissues, no previous studies have evaluated amino acids or lipids as potential chronic prostatitis biomarkers.

Objectives

The study’s aim was to identify amino acids and lipids that could serve as potential biomarkers of chronic prostatitis.

Methods

We profiled the amino acids and lipids found in plasma from rats collected in a previous study. In brief, a total of 148 Sprague–Dawley rats (offspring) were dosed with estradiol benzoate (EB) on postnatal days (PNDs) 1, 3 and 5, and subsequently dosed with testosterone (T)/estradiol (E) tubes via subcutaneous implants from PND 90 to 200. Plasma was collected on PNDs 30, 90, 100, 145 and 200. Analysis was conducted with a Xevo TQ-S triple-quadrupole mass spectrometer using a Biocrates AbsoluteIDQ p180 kit.

Results

Plasma acylcarnitines [(C2, C16:1, C18, C18:1, C18:1-OH, and C18:2)], glycerophospholipids (lysophosphatidylcholine-acyl, -di-acyl, and -di-acyl acyl-alkyl) and sphingomyelins [SM (OH) C16:1, SM C18:0, SM C18:1, and SM C20:2] significantly increased on PND 145, when chronic inflammation was observed in the dorsolateral prostate of rats dosed with EB, T, and E. No statistical significances of amino acid levels were observed in the EB + T + E group on PND 145.

Conclusion

Exposure to EB, T, and E altered lipid levels in rat plasma with chronic prostate inflammation. These findings suggest that the identified lipids may be predictive chronic prostatitis biomarkers. The results require confirmation through additional nonclinical and human studies.

中文翻译：

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在苯甲酸雌二醇和性激素引起的慢性炎症大鼠血浆中鉴定出明显的脂质特征。

介绍

前列腺炎可能发生在年轻或中年男性中，而前列腺癌则可能发生在老年男性中。尽管已经使用前列腺癌细胞系/组织检查了作为前列腺癌生物标志物的氨基酸和脂质，但是以前没有研究评估氨基酸或脂质作为潜在的慢性前列腺炎生物标志物。

目标

该研究的目的是鉴定可作为慢性前列腺炎潜在生物标志物的氨基酸和脂质。

方法

我们对先前研究中收集的大鼠血浆中发现的氨基酸和脂质进行了分析。简而言之，在出生后第1、3和5天给总共148只Sprague-Dawley大鼠（后代）服用苯甲酸雌二醇（EB），然后通过皮下注射睾丸激素（T）/雌二醇（E）管植入PND 90至200。将血浆收集在PND 30、90、100、145和200上。使用Xevo TQ-S三重四极杆质谱仪使用Biocrates AbsoluteIDQ p180试剂盒进行分析。

结果

血浆酰基肉碱[（C2，C16：1，C18，C18：1，C18：1-OH和C18：2）]，甘油磷脂（溶血磷脂酰胆碱-酰基，-二-酰基和-二-酰基酰基-烷基）和鞘磷脂[SM（OH）C16：1，SM C18：0，SM C18：1和SM C20：2]在PND 145上显着增加，当在用EB，T和E给药的大鼠的背外侧前列腺中观察到慢性炎症时E.在PND 145的EB + T + E组中未观察到氨基酸水平的统计学意义。

结论

暴露于EB，T和E会改变患有慢性前列腺炎的大鼠血浆中的脂质水平。这些发现表明，所鉴定的脂质可能是预测性慢性前列腺炎的生物标志物。结果需要通过其他非临床和人体研究来证实。