Late blight is a disease with the biggest economic impact on potato cultivation worldwide. Pyramiding of the resistance genes originating from potato wild relatives is a breeding strategy that has a potential to produce potato cultivars durably resistant to late blight. Growing such cultivars would allow limiting the intensive chemical control of the disease. The goal of this work was to transfer the late blight resistance gene Rpi-rzc1 from Solanum ruiz-ceballosii to the tetraploid level of cultivated potato and to pyramid it with the Rpi-phu1 gene. We obtained two diploid and, through 4x-2x cross, a tetraploid potato population segregating for the Rpi-rzc1 presence, as well as one diploid and one tetraploid population where both genes were introgressed. In total, 754 progeny clones were tested for resistance to late blight in detached leaflet assays. Pathogen isolates avirulent on plants with both genes and virulent on plants with the Rpi-phu1 were used. The selection was assisted by two PCR markers flanking the Rpi-rzc1 gene and a newly designed, highly specific intragenic marker indicating the Rpi-phu1 gene presence. We obtained 26 diploid and 49 tetraploid potato clones with pyramid of both genes that should enhance the durability and spectrum of their late blight resistance and that can be exploited in potato breeding. The specificity of the marker for the Rpi-phu1 gene and the precision of the Rpi-rzc1 mapping were improved in this work.

晚疫病是一种对全球马铃薯种植产生最大经济影响的疾病。起源于马铃薯野生近缘种的抗性基因的金字塔化是一种育种策略，它有可能产生对晚疫病具有持久抗性的马铃薯品种。生长这样的品种将限制对疾病的严格化学控制。这项工作的目的是将晚疫病抗性基因Rpi-rzc1从茄子（Solaum ruiz-ceballosii）转移到栽培马铃薯的四倍体水平，并与Rpi-phu1基因形成金字塔。我们获得了两个二倍体，并通过4x-2x杂交，分离了一个四倍体马铃薯种群，用于Rpi-rzc1以及两个基因都渗入的一个二倍体和一个四倍体种群。在分离的小叶测定中，总共测试了754个后代克隆对晚疫病的抗性。使用对两种基因均无毒且对Rpi-phu1具有毒性的病原菌。通过两个Rpi-rzc1基因侧翼的PCR标记和一个新设计的高度特异性的基因内标记来辅助选择，表明Rpi-phu1基因的存在。我们获得了具有两个基因金字塔的26个二倍体和49个四倍体马铃薯克隆，这两个基因应增强其晚疫病抗性的持久性和频谱，并且可以在马铃薯育种中加以利用。Rpi-phu1标记的特异性这项工作提高了Rpi-rzc1基因的基因和定位精度。