The use of microorganisms capable of mediating the bioprecipitation process can be an important application in the self-healing processes of cement specimens. Thus, the present study identified and evaluated five Bacillus strains for potential application in the protocol of self-healing via bioprecipitation. Cell growth, enzyme production, and kinetic parameters conditions were evaluated during the fermentation process. Based on the analysis of 16S rDNA in conjunction with biochemical testing, results demonstrate that the strains are either Bacillus cereus or Bacillus thuringiensis. Strategically it was found that the addition of glycerol to fermentative medium was essential to increase the bacterial concentration (≈ 4.2 × 10⁷ cells mL⁻¹) and production of the enzyme urease (≈ 3.623,2 U.mL⁻¹). The addition of this medium after 40 days of fermentation promoted the self-healing of cracks and increased compressive strength in ≈ 14.2% of the cementitious specimens; therefore, increasing the sustainability and engineering properties of cement-based materials.

能够介导生物沉淀过程的微生物的使用可以成为水泥样品自愈过程的重要应用。因此，本研究鉴定并评估了五种芽孢杆菌菌株，它们在通过生物沉淀自愈方案中的潜在应用。在发酵过程中评估细胞生长、酶生产和动力学参数条件。根据16S rDNA分析和生化检测，结果表明菌株为蜡样芽孢杆菌或苏云金芽孢杆菌。从策略上发现，向发酵培养基中添加甘油对于增加细菌浓度（≈ 4.2 × 10 ⁷细胞 mL ^-1 ) 和酶脲酶的产生 (≈ 3.623,2 U.mL ^-1 )。在发酵 40 天后添加这种培养基促进了裂缝的自愈并增加了约 14.2% 的水泥试样的抗压强度；因此，提高了水泥基材料的可持续性和工程性能。