The study was aimed to develop rust resistant genotypes with genome of prominent wheat variety HS240 by pyramiding of linked leaf rust and stem rust resistance genes Lr19 and Sr25 with stripe rust resistance gene Yr15. The genotypes FLW13 and FLW20 were used as gene donors for transfer of Yr15 and Lr19/Sr25, respectively. The molecular markers scs265, wmc221, PSY1-E1, and Gb linked to Lr19/Sr25 and barc8 and gwm11 linked to Yr15 were used for foreground selection. Background selection, involving 58 simple sequence repeat (SSR) markers polymorphic between HS240 and FLW20; 72 between HS240 and FLW13 was carried out to recover the genome of HS240 in advanced bulks derived from a cross HS240*2/FLW20//HS240*2/FLW13. The SSR based genome recovery in selected advanced breeding lines, WBM3682 and WBM3684 was 90.8% and 93.3%, respectively. The 35 K SNP array based analysis of genomic regions of HS240 also substantiated the results of genomic recovery estimated through SSR markers. The selected line WBM3682 recorded average grain yield of 25.7q/ha and showed its superiority over parental check HS240 under rainfed situations. This study has led to development of rust resistant wheat carrying genome of HS240.

中文翻译：

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通过在小麦 (Triticum aestivum L.) 中将 Lr19/Sr25 和 Yr15 金字塔化，分子标记辅助选择开发抗锈病基因型

本研究旨在通过将连锁叶锈病和茎锈病抗性基因 Lr19 和 Sr25 与条锈病抗性基因 Yr15 进行金字塔式叠加，开发具有主要小麦品种 HS240 基因组的抗锈病基因型。基因型 FLW13 和 FLW20 分别用作 Yr15 和 Lr19/Sr25 转移的基因供体。分子标记 scs265、wmc221、PSY1-E1 和与 Lr19/Sr25 连锁的 Gb 和与 Yr15 连锁的 barc8 和 gwm11 用于前景选择。背景选择，涉及 HS240 和 FLW20 之间多态性的 58 个简单序列重复 (SSR) 标记；在 HS240 和 FLW13 之间进行 72 以在源自交叉 HS240*2/FLW20//HS240*2/FLW13 的高级批量中恢复 HS240 的基因组。在选定的先进育种品系 WBM3682 和 WBM3684 中，基于 SSR 的基因组回收率分别为 90.8% 和 93.3%。基于 35 K SNP 阵列的 HS240 基因组区域分析也证实了通过 SSR 标记估计的基因组恢复结果。所选品系 WBM3682 的平均籽粒产量为 25.7q/ha，在雨养条件下表现出优于亲本检查 HS240。该研究导致开发了携带 HS240 基因组的抗锈小麦。