A new metal coordination complex [Zn(L)(N3)]4 (1) based on the Schiff base ligand 2-ethoxy-6-methyliminomethyl-phenol (HL) is successfully prepared via a slow evaporation method. The as-prepared metal complex is characterized by various physicochemical techniques, e.g. elemental analysis and X-ray single crystal diffraction. Furthermore, we use a mechanical grinding method to prepare nanosized crystalline complex 1 (nano-1). The anti-liver cancer activity of nano-1 is evaluated in vitro. Firstly, the cell viability and cell proliferation ability are assessed with CCK-8 and cell colony formation assay, respectively. In addition to this, the cell migration and invasion are also evaluated with the transwell assay after indicated treatment.

中文翻译：

---

2-乙氧基-6-甲基亚胺甲基-苯酚的新型四核锌络合物的晶体结构和抗肝癌活性

通过缓慢蒸发方法成功制备了一种基于席夫碱配体 2-乙氧基-6-甲基亚氨基甲基苯酚 (HL) 的新型金属配位配合物 [Zn(L)(N3)]4 (1)。所制备的金属配合物的特征在于各种物理化学技术，例如元素分析和 X 射线单晶衍射。此外，我们使用机械研磨方法制备纳米晶体复合物 1 (nano-1)。nano-1 的抗肝癌活性在体外进行了评估。首先，分别用CCK-8和细胞集落形成试验评估细胞活力和细胞增殖能力。除此之外，在指定处理后，还使用 ​​transwell 测定评估细胞迁移和侵袭。