Iron oxide nanoparticles (IONPs) were synthesized from the Proteus vulgaris ATCC-29905 using the extracellular methodology. The UV–Vis spectrophotometry showed the λ-max (maximum absorbance) absorption peak at 310 nm. The FTIR analysis showed amides and other functional groups are associated with IONPs. The TGA results showed less protein loss (ΔY = 9.045%) at a protein degradation temperature of 71.73 °C. The FESEM images showed particles are spherical in shape. The EDX confirms the presence of iron. Zeta potential interface was found to be 79.5 mV that confirms its stability. The TEM results showed particles are spherical with a diameter between 19.23 nm ad 30.51 nm. These IONPs showed good antibacterial activity and also showed good activity against methicillin resistant staphylococcus aureus (MRSA). It showed good antioxidant activity. These IONPs exhibits good cytotoxic effect against U87 MG—glioblastoma cancer cells, showed IC50 value at 250 µg/ml compared with healthy L-132 cells. Scratch assay showed IONPs inhibit the cell migration of the HT-29 cancer cells. The nanoparticles synthesized from bacteria are safe and non-hazardous. It was expected that these IONPs could become a potential anticancer and antibacterial agent, and in the future it opens a new path for treating the cancer patients.

使用细胞外方法，从变形杆菌ATCC- 29905合成了氧化铁纳米颗粒（IONP）。紫外可见分光光度法显示在310 nm处有一个λ-max（最大吸收）吸收峰。FTIR分析表明酰胺和其他官能团与IONP相关。TGA结果显示，在71.73°C的蛋白质降解温度下，蛋白质损失较少（ΔY= 9.045％）。FESEM图像显示颗粒为球形。EDX证实了铁的存在。发现Zeta电位界面为79.5 mV，证实了其稳定性。TEM结果表明，颗粒是球形的，直径在19.23nm至30.51nm之间。这些IONPs表现出良好的抗菌活性，还显示出对耐甲氧西林金黄色葡萄球菌的良好活性（MRSA）。显示出良好的抗氧化活性。这些IONP对U87 MG-胶质母细胞瘤癌细胞显示出良好的细胞毒性作用，与健康的L-132细胞相比，IC50值为250 µg / ml。刮擦试验表明，IONPs抑制HT-29癌细胞的细胞迁移。由细菌合成的纳米颗粒是安全且无害的。预期这些IONPs可能成为潜在的抗癌和抗菌剂，并且在将来，它为治疗癌症患者开辟了一条新途径。