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Bacteria Mediated Synthesis of Iron Oxide Nanoparticles and Their Antibacterial, Antioxidant, Cytocompatibility Properties

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Abstract

Iron oxide nanoparticles (IONPs) were synthesized from the Proteus vulgaris ATCC-29905 using the extracellular methodology. The UV–Vis spectrophotometry showed the λ-max (maximum absorbance) absorption peak at 310 nm. The FTIR analysis showed amides and other functional groups are associated with IONPs. The TGA results showed less protein loss (ΔY = 9.045%) at a protein degradation temperature of 71.73 °C. The FESEM images showed particles are spherical in shape. The EDX confirms the presence of iron. Zeta potential interface was found to be 79.5 mV that confirms its stability. The TEM results showed particles are spherical with a diameter between 19.23 nm ad 30.51 nm. These IONPs showed good antibacterial activity and also showed good activity against methicillin resistant staphylococcus aureus (MRSA). It showed good antioxidant activity. These IONPs exhibits good cytotoxic effect against U87 MG—glioblastoma cancer cells, showed IC50 value at 250 µg/ml compared with healthy L-132 cells. Scratch assay showed IONPs inhibit the cell migration of the HT-29 cancer cells. The nanoparticles synthesized from bacteria are safe and non-hazardous. It was expected that these IONPs could become a potential anticancer and antibacterial agent, and in the future it opens a new path for treating the cancer patients.

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Acknowledgements

The authors would like to thank Universiti Kuala Lumpur Royal College of medicine for providing the necessary facilities and support in this study. I would also like to thank the school of chemical sciences university Sains Malaysia for their essential support to carry out this study.

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Authors and Affiliations

  1. Faculty of Pharmacy and Health Sciences, Universiti Kuala Lumpur Royal College of Medicine Perak, 30450, Ipoh, Perak, Malaysia

    Shahnaz Majeed

  2. Bioengineering Technology Section, Universiti Kuala Lumpur Malaysian, Institute of Chemical and Bioengineering Technology (MICET), Lot 1988, Kawasan Perindustrian Bandar Vendor, Taboh Naning, 78000, Alor Gajah, Melaka, Malaysia

    Mohammed Danish

  3. School of Chemical Sciences, Universiti Sains Malaysia, 11800, Penang, Malaysia

    Mohamad Nasir Mohamad Ibrahim & Siti Hajar Sekeri

  4. School of Pharmacy, University of Nottingham Malaysia, 43500, Semenyih, Malaysia

    Mohammed Tahir Ansari

  5. Department of Biomedical Engineering, Sathyabama University, Chennai, India

    Anima Nanda & Gulzar Ahmad

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  1. Shahnaz Majeed
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Majeed, S., Danish, M., Mohamad Ibrahim, M.N. et al. Bacteria Mediated Synthesis of Iron Oxide Nanoparticles and Their Antibacterial, Antioxidant, Cytocompatibility Properties. J Clust Sci 32, 1083–1094 (2021). https://doi.org/10.1007/s10876-020-01876-7

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