Carbon source plays an important role in the induction of embryogenic callus (EC). Therefore it is necessary to determine the effective carbohydrate for improving this process. In this study, different carbohydrates as sucrose, maltose, and sorbitol with different levels (0–30 g/L) were tested in the callus induction of Pyrus communis. At first, in solid culture, calli were divided in relation to their morphological appearance in EC and non-EC. EC was white or yellow with the granular aspect. The morphological study revealed the highest frequency of EC (28.5%) obtained at 30 g/L sucrose. Biochemical analyses showed EC exhibited both soluble sugar and protein high contents, while the starch was at the lowest level. As well as flow cytometry showed EC were genetically similar to donor explants. With transferring the EC into the liquid culture, then, subculturing calli along with 2 ml cell suspension on solid medium led to the globular embryo.

碳源在诱导胚性愈伤组织（EC）中起重要作用。因此，有必要确定用于改善该过程的有效碳水化合物。在这项研究中，测试了不同的碳水化合物（如蔗糖，麦芽糖和山梨糖醇）的含量（0–30 g / L），并对其诱导的梨属愈伤组织进行了测试。。首先，在固体培养中，愈伤组织根据其在EC和非EC中的形态出现而被划分。EC为白色或黄色，颗粒状。形态研究表明，蔗糖浓度为30 g / L时，EC的最高频率（28.5％）。生化分析表明，EC显示出高含量的可溶性糖和蛋白质，而淀粉处于最低水平。流式细胞仪显示EC在遗传上与供体外植体相似。通过将EC转移到液体培养物中，然后将愈伤组织与2 ml细胞悬浮液一起在固体培养基上继代培养，形成了球形胚。