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The Role of Carbohydrates on The Induction of Somatic Embryogenesis and The Biochemical State of The Embryogenic Callus in Pyrus communis L. Cv. ‘Dar Gazi’

Die Rolle von Kohlenhydraten bei der Induktion der somatischen Embryogenese und der biochemischen Beschaffenheit des embryogenen Kallusgewebes von Pyrus communis L. cv. ’Dar Gazi’

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Abstract

Carbon source plays an important role in the induction of embryogenic callus (EC). Therefore it is necessary to determine the effective carbohydrate for improving this process. In this study, different carbohydrates as sucrose, maltose, and sorbitol with different levels (0–30 g/L) were tested in the callus induction of Pyrus communis. At first, in solid culture, calli were divided in relation to their morphological appearance in EC and non-EC. EC was white or yellow with the granular aspect. The morphological study revealed the highest frequency of EC (28.5%) obtained at 30 g/L sucrose. Biochemical analyses showed EC exhibited both soluble sugar and protein high contents, while the starch was at the lowest level. As well as flow cytometry showed EC were genetically similar to donor explants. With transferring the EC into the liquid culture, then, subculturing calli along with 2 ml cell suspension on solid medium led to the globular embryo.

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Authors and Affiliations

  1. Department of Horticultural Science and Landscape, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran

    Atefe Ameri, Gholam Hossein Davarynejad & Ali Tehranifar

  2. Department of Biotechnology and Plant Breeding, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran

    Nasrin Moshtaghi

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  1. Atefe Ameri
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  2. Gholam Hossein Davarynejad
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  3. Nasrin Moshtaghi
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  4. Ali Tehranifar
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Correspondence to Gholam Hossein Davarynejad.

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A. Ameri, G.H. Davarynejad, N. Moshtaghi and A. Tehranifar declare that they have no competing interests.

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Ameri, A., Davarynejad, G.H., Moshtaghi, N. et al. The Role of Carbohydrates on The Induction of Somatic Embryogenesis and The Biochemical State of The Embryogenic Callus in Pyrus communis L. Cv. ‘Dar Gazi’. Erwerbs-Obstbau 62, 411–419 (2020). https://doi.org/10.1007/s10341-020-00518-6

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  • DOI: https://doi.org/10.1007/s10341-020-00518-6

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