The bacteriophage vB_AhM_PVN02 (PVN02), infecting Aeromonas hydrophila, was isolated from a striped catfish pond water sample in Can Tho City, Vietnam. The phage had high lytic activity with a latent period and burst size of approximately 20 min and 105 plaque-forming units per cell, respectively. Observation of the phage by transmission electron microscopy indicated that PVN02 belongs to the family Myoviridae. The genome of PVN02 is a double-stranded linear DNA with a length in 51,668 bp and a content of 52% GC. Among the 64 genes, 16 were predicted to encode proteins with predicted functions. No virulence or antibiotic resistance genes were found in the genome, suggesting it would be a useful biocontrol agent. Classification of the phage based on sequence comparisons, phylogenetic analysis, and gene-sharing networks was carried out, and it was found to be the first representative of a new species within a previously undefined genus in the family Myoviridae. This study confirmed that PVN02 is a novel lytic phage that could potentially be used as an agent to control Aeromonas hydrophila in striped catfish in the Mekong Delta, Vietnam.

感染嗜水气单胞菌的噬菌体vB_AhM_PVN02（PVN02）是从越南芹T市的一条条纹cat鱼池塘水样本中分离出来的。该噬菌体具有高的裂解活性，潜伏期和破裂大小分别约为每个细胞20分钟和105个噬菌斑形成单位。通过透射电子显微镜观察噬菌体表明PVN02属于肌病毒科。。PVN02的基因组是双链线性DNA，长度为51,668 bp，GC含量为52％。在这64个基因中，有16个被预测为编码具有预测功能的蛋白质。在基因组中未发现任何毒性或抗生素抗性基因，表明它将是有用的生物防治剂。根据序列比较，系统发育分析和基因共享网络对噬菌体进行了分类，发现它是肌病毒科以前未定义属中新物种的第一个代表。这项研究证实，PVN02是一种新型的裂解噬菌体，可以潜在地用作控制湄公河三角洲条纹striped鱼中嗜水气单胞菌的药剂。