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Complete genome sequence of a novel lytic phage infecting Aeromonas hydrophila, an infectious agent in striped catfish (Pangasianodon hypophthalmus)

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Abstract

The bacteriophage vB_AhM_PVN02 (PVN02), infecting Aeromonas hydrophila, was isolated from a striped catfish pond water sample in Can Tho City, Vietnam. The phage had high lytic activity with a latent period and burst size of approximately 20 min and 105 plaque-forming units per cell, respectively. Observation of the phage by transmission electron microscopy indicated that PVN02 belongs to the family Myoviridae. The genome of PVN02 is a double-stranded linear DNA with a length in 51,668 bp and a content of 52% GC. Among the 64 genes, 16 were predicted to encode proteins with predicted functions. No virulence or antibiotic resistance genes were found in the genome, suggesting it would be a useful biocontrol agent. Classification of the phage based on sequence comparisons, phylogenetic analysis, and gene-sharing networks was carried out, and it was found to be the first representative of a new species within a previously undefined genus in the family Myoviridae. This study confirmed that PVN02 is a novel lytic phage that could potentially be used as an agent to control Aeromonas hydrophila in striped catfish in the Mekong Delta, Vietnam.

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Acknowledgements

This research was funded by the Vietnam National Foundation for Science and Technology Development (NAFOSTED) under grant number 10/2019/TN.

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Authors and Affiliations

  1. Faculty of Chemical Engineering, Ho Chi Minh City University of Technology (HCMUT), 268 Ly Thuong Kiet, District 10, Ho Chi Minh City, Vietnam

    Vinh Q. Tu, Tan-Trung Nguyen, Xuan T. T. Tran, Huyen T. Phan, Nga P. Le & Hoang A. Hoang

  2. Vietnam National University Ho Chi Minh City, Linh Trung Ward, Thu Duc District, Ho Chi Minh City, Vietnam

    Vinh Q. Tu, Tan-Trung Nguyen, Xuan T. T. Tran, Huyen T. Phan, Nga P. Le & Hoang A. Hoang

  3. Institut Jean-Pierre Bourgin INRAE, AgroParisTech, CNRS, Université Paris-Saclay, 78000, Versailles, France

    Tan-Trung Nguyen

  4. Department of Genetics and Genome Biology, University of Leicester, University Road, Leicester, UK

    Andrew D. Millard

  5. College of Aquaculture and Fisheries, Can Tho University, Campus II, 3/2 St., Ninh Kieu District, Can Tho, Vietnam

    Oanh T. H. Dang

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  1. Vinh Q. Tu
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  2. Tan-Trung Nguyen
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  3. Xuan T. T. Tran
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  4. Andrew D. Millard
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  5. Huyen T. Phan
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  7. Oanh T. H. Dang
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  8. Hoang A. Hoang
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Correspondence to Hoang A. Hoang.

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Tu, V.Q., Nguyen, TT., Tran, X.T.T. et al. Complete genome sequence of a novel lytic phage infecting Aeromonas hydrophila, an infectious agent in striped catfish (Pangasianodon hypophthalmus). Arch Virol 165, 2973–2977 (2020). https://doi.org/10.1007/s00705-020-04793-2

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  • DOI: https://doi.org/10.1007/s00705-020-04793-2

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