Abstract

A micelle mediated separation and preconcentration method for flame atomic absorption spectrophotometric (FAAS) determination of Cu was developed. The method is based on complex formation between Cu and 1-(2-pyridylazo)-2-naphthol and cloud point extraction with Tergitol NP-7 surfactant prior to FAAS determination. The parameters affecting quantitative extraction of copper, including pH, surfactant and ligand concentrations, incubation time and temperature, were examined and optimized. Under optimum conditions, interference effects of other ions were studied, and tolerance ratios of interference ions were given. Under the optimal conditions, detection and quantitation limits were found as 3 and 8 μg/L, respectively. Linear dynamic range for Cu²⁺ was determined to be 0.13–1.5 μg/mL. Relative standard deviations were below 6% throughout the experiments. Enhancement factor was determined as 25. Applicability of the method was proved by analysis of standard reference materials. The developed method was applied to determine trace copper contents in different mushroom species.

中文翻译：

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胶束介导萃取和火焰原子吸收光谱法测定不同菌种中的痕量铜

摘要

建立了胶束介导的分离富集火焰原子吸收分光光度法（FAAS）测定铜的方法。该方法基于铜与1-（2-吡啶基偶氮）-2-萘酚之间的络合物形成，以及在FAAS测定之前用Tergitol NP-7表面活性剂萃取浊点。检查并优化了影响铜定量提取的参数，包括pH，表面活性剂和配体浓度，孵育时间和温度。在最佳条件下，研究了其他离子的干扰效应，并给出了干扰离子的容忍度。在最佳条件下，检测限和定量限分别为3和8μg/ L。Cu ^2+的线性动态范围经测定为0.13–1.5μg/ mL。在整个实验中，相对标准偏差均低于6％。确定增强因子为25。通过分析标准参考物质证明了该方法的适用性。将该方法用于测定不同菌种中痕量铜的含量。