To elucidate the individual and multiple roles of physiological bacterial groups involved in biogeochemical cycles of carbon, nitrogen, phosphorus and sulfur, the changes in the abundance of aerobic bacteria (heterotrophs, methane oxidizers, ammonia oxidizers, sulfur oxidizers, phosphate solubilizers, phosphate accumulators) and anaerobic bacteria (total anaerobes, nitrate reducers, denitrifiers and sulfate reducers) were investigated in a biosecured, zero-exchange system stocked with whiteleg shrimp, Litopenaeus vannamei for one production cycle. Key water quality parameters during the 96-day production cycle fell within the normal range for L. vannamei culture. Results of Spearman’s correlation matrix revealed that different sets of variables correlated at varying levels of significance of the interrelationships between bacterial abundances and water quality parameters. The three nitrogenous species (ammonia, nitrite and nitrate) strongly influenced the physiological bacterial groups’ abundance. The strong relationship of bacterial groups with phytoplankton biomass and abundance clearly showed the trophic interconnections in nutrient exchange/recycling. Canonical correspondence analysis performed to assess the total variation revealed that the three dissolved nitrogen species followed by salinity, temperature, phytoplankton biomass and pH collectively accounted for as much as 82% of the total variation. In conclusion, the results of the study revealed that the major drivers that interweaved biogeochemical cycles are the three dissolved nitrogen species, which microbially mediated various aerobic-anaerobic assimilation/dissimilation processes in the pond ecosystem. Considering the pond microbial ecology becoming an important management tool where applied research could improve the economic and environmental sustainability of the aquaculture industry, the findings of the present study are practically relevant.

为了阐明参与碳，氮，磷和硫的生物地球化学循环的生理细菌群的个体和多重作用，需氧细菌（异养菌，甲烷氧化剂，氨氧化剂，硫氧化剂，磷酸盐增溶剂，磷酸盐蓄能器）的丰度变化在一个生物安全，零交换的系统中，对白斑虾南美白对虾进行了一个生产周期的研究，研究了厌氧细菌（总厌氧菌，硝酸盐还原剂，反硝化剂和硫酸盐还原剂）的总含量。96天生产周期中的关键水质参数均在南美白对虾的正常范围内文化。Spearman相关矩阵的结果表明，不同的变量集在细菌丰度与水质参数之间的相互关系的显着性水平上具有相关性。三种含氮物质（氨，亚硝酸盐和硝酸盐）强烈影响生理细菌群体的丰度。细菌群与浮游植物生物量和丰富度之间的密切关系清楚地表明了养分交换/再循环中的营养联系。进行标准对应分析以评估总变化，发现三种溶解氮种类，其次为盐度，温度，浮游植物生物量和pH，总计占总变化的82％。结论，研究结果表明，交织生物地球化学循环的主要驱动力是三种溶解氮，它们通过微生物介导了池塘生态系统中各种好氧-厌氧的同化/异化过程。考虑到池塘微生物生态学已成为一种重要的管理工具，应用研究可以改善水产养殖业的经济和环境可持续性，因此本研究的发现实际上具有现实意义。