Recently, the production of exopolysaccharide from Pseudozyma sp. SY16, which is mannosylerythritol lipid-producing yeast, has been occasionally observed during industrial fermentations. Moreover, exopolysaccharides derived from microbes are in high demand for the production of biopolymers. Therefore, this study aimed to investigate the production and characteristics of the exopolysaccharide produced by this strain. In batch fermentations with this strain, the maximum production yield of exopolysaccharide (5.8 g/L) was obtained under olive oil supplementation as a carbon source with culture conditions of 30°C, pH 8.0, and 600 rpm. Through a series of liquid chromatography steps, the exopolysaccharide was successfully purified, and its emulsifying activity was demonstrated. In addition, two-dimensional nuclear magnetic resonance analysis revealed that the exopolysaccharide is a galactoglucomannan with a novel and unique structure, consisting of galactose, glucose, and mannose in the ratio of 1:5:3.

最近，从假酶生产胞外多糖sp。SY16是生产甘露糖基赤藓糖醇的脂质酵母，在工业发酵过程中偶尔会观察到。此外，源自微生物的胞外多糖对生产生物聚合物的需求很高。因此，本研究旨在研究该菌株产生的胞外多糖的产生及其特性。在此菌株的分批发酵中，在橄榄油为碳源，培养条件为30°C，pH 8.0和600 rpm的条件下，获得了最大多糖产量（5.8 g / L）。通过一系列的液相色谱步骤，成功地纯化了胞外多糖，并证明了其乳化活性。此外，