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A Novel Galactoglucomannan Exopolysaccharide Produced by Oil Fermentation with Pseudozyma sp. SY16

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Abstract

Recently, the production of exopolysaccharide from Pseudozyma sp. SY16, which is mannosylerythritol lipid-producing yeast, has been occasionally observed during industrial fermentations. Moreover, exopolysaccharides derived from microbes are in high demand for the production of biopolymers. Therefore, this study aimed to investigate the production and characteristics of the exopolysaccharide produced by this strain. In batch fermentations with this strain, the maximum production yield of exopolysaccharide (5.8 g/L) was obtained under olive oil supplementation as a carbon source with culture conditions of 30°C, pH 8.0, and 600 rpm. Through a series of liquid chromatography steps, the exopolysaccharide was successfully purified, and its emulsifying activity was demonstrated. In addition, two-dimensional nuclear magnetic resonance analysis revealed that the exopolysaccharide is a galactoglucomannan with a novel and unique structure, consisting of galactose, glucose, and mannose in the ratio of 1:5:3.

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Acknowledgements

This research was supported by the KRIBB (Korea Research Institute of Bioscience and Biotechnology) Research Initiative Program (Grant No. KGS1292012; KGM5252012)(http://www.kribb.re.kr).

The authors declare no conflict of interest.

Neither ethical approval nor informed consent was required for this study.

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Author notes

  1. Kwang Nyeon Kim and Yong Jae Lee contributed equally to this work

Authors and Affiliations

  1. R&D Center, United Active Inc., Seoul, 08381, Korea

    Kwang Nyeon Kim

  2. Cell Factory Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, 34141, Korea

    Yong Jae Lee, Dae-Hyun Cho, Keug-Hyun Ahn & Hee-Sik Kim

  3. DKBio Ltd., Daejeon, 34141, Korea

    Jong-Eon Hong & Hee-Sik Kim

  4. Department of Biotechnology and The Research Centre for Biopharmaceutical Lead Molecule (GRRC), The Catholic University of Korea, Bucheon, 14662, Korea

    Yong-Il Park

  5. Major of Environmental Biotechnology, University of Science & Technology (UST), Daejeon, 34113, Korea

    Hee-Sik Kim

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  1. Kwang Nyeon Kim
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Correspondence to Hee-Sik Kim.

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Kim, K.N., Lee, Y.J., Cho, DH. et al. A Novel Galactoglucomannan Exopolysaccharide Produced by Oil Fermentation with Pseudozyma sp. SY16. Biotechnol Bioproc E 25, 742–748 (2020). https://doi.org/10.1007/s12257-020-0066-x

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  • DOI: https://doi.org/10.1007/s12257-020-0066-x

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