Two cases of coinfection with bovine papular stomatitis virus (BPSV) and pseudocowpox virus (PCPV) in dairy calves in Tochigi Prefecture, Japan, are reported. Sequences of BPSV and PCPV were simultaneously detected in the same polymerase chain reaction (PCR) amplicons, which were obtained from the DNA of two dairy calves using a pan-parapoxvirus primer set. PCR amplification using BPSV- and PCPV-specific primer sets were able to distinguish between the two viruses in coinfected clinical samples. Based on these data, further studies on the occurrence BPSV/PCPV coinfections in cattle in Japan are warranted.

中文翻译：

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日本乳牛犊中的牛丘疹性口腔炎病毒和伪牛痘病毒并发感染。

据报道，在日本To木县的牛犊中，发生了两例牛丘疹性口炎病毒（BPSV）和伪牛痘病毒（PCPV）的合并感染。在同一聚合酶链反应（PCR）扩增子中同时检测到BPSV和PCPV的序列，这些扩增子是使用泛副痘病毒引物组从两个乳牛的DNA中获得的。使用BPSV特异性和PCPV特异性引物对的PCR扩增能够区分共感染临床样品中的两种病毒。基于这些数据，有必要对日本牛中BPSV / PCPV合并感染的发生进行进一步研究。