The present study evaluated the efficacy of swine vacciation using a combination of mixable monovalents for porcine circovirus type 2 (PCV2) and Mycoplasma hyopneumoniae against a ready-to-use bivalent vaccine under experimental conditions. Pigs at 21 days of age were administered either a combination of two mixable monovalent vaccines or a bivalent vaccine containing PCV2 and M. hyopneumoniae. Vaccination was followed with an M. hyopneumoniae challenge at 42 days of age (−14 days post challenge, dpc) and a PCV2d challenge at 56 days of age (0 dpc). Each vaccinated and challenged group was compared with the unvaccinated and challenged group for clinical, microbiological, immunologic, and pathologic differences. Clinically, two vaccinated and challenged groups showed minimal respiratory diseases that was characterized by occasionally coughing and sneezing. A significant difference was not calculated in the average daily weight gain, nasal shedding of M. hyopneumoniae, and pathological lesions between two vaccinated and challenged groups. A combination of two monovalent vaccines mixed into a combo prior to vaccination followed by challenge resulted in increased numbers of PCV2d-specific interferon-γ secreting cells at 21 dpc and a significant reduction in PCV2d viremia at 14 dpc when compared with the ready-to-use bivalent-vaccinated and challenged groups. These results offer supporting evidence that vaccination during the weaning to finishing period against M. hyopneumoniae and PCV2 is efficacious for controlling diseases caused by these two pathogens.

本研究评估了猪圆环病毒2型（PCV2）和猪圆环病毒的混合单价疫苗接种的有效性 猪肺炎支原体在实验条件下针对现成的二价疫苗。给21日龄的猪接种两种可混合的单价疫苗或含有PCV2和猪肺炎支原体。接种疫苗后猪肺炎支原体在42天的年龄（挑战后-14天，dpc）进行挑战，在56天的年龄（0 dpc）进行PCV2d挑战。将每个接种和攻击的组与未接种和攻击的组进行临床，微生物学，免疫学和病理学差异的比较。临床上，两个接种疫苗和攻击的人群表现出极少的呼吸系统疾病，其特征是偶尔咳嗽和打喷嚏。没有计算出平均每日体重增加，鼻腔脱落的显着差异。猪肺炎支原体，以及两个接种和攻击组之间的病理病变。与现成疫苗相比，两种单价疫苗在接种疫苗之前先混合后接种疫苗，然后再进行攻击，导致PCV2d特异性干扰素-γ分泌细胞的数量在21 dpc时增加，在14 dpc时PCV2d病毒血症显着降低。使用二价疫苗接种和攻击的人群。这些结果提供了支持性证据，表明在断奶至结束期间接种猪肺炎支原体 PCV2可有效控制这两种病原体引起的疾病。