OBJECTIVES The study was designed to determine the presence of Helicobacter genus and three species of H. pylori, H. bilis, and H. canis, in the duodenum, ileum, colon, and liver of stray cats. Moreover, the histopathological and immunohistochemical analyses have been performed. METHODS Samples were taken from the duodenum, ileum, colon, and liver of 30 cats for molecular and histopathological evaluations. Polymerase chain reaction was carried out for the detection of the Helicobacter genus in the mentioned samples. Then, species-specific primers were used in Helicobacter-positive samples. RESULTS Helicobacter genus prevalence rates in the duodenum, ileum, colon, and liver samples were 50%, 60%, 50%, and 43.3%, respectively. Helicobacter pylori, H. canis, and H. bilis were isolated from at least one tissue of 18 (60%), 13 (43.3%), and 8 (26.7%) of the cats, respectively. Immunohistochemical findings confirmed the presence of bacteria in the intestinal crypt or the mucosal layer of duodenum, ileum, colon, and hepatic sinusoids. CONCLUSION In the present study, the concurrent infection of duodenum and liver was noticeable. Furthermore, the high prevalence of H. pylori in cats, as a well-known human pathogen, should be considered. High incidence of Helicobacter in gut and liver of Ahvaz stray cats is noticeable. According to the zoonotic importance of Helicobacter, more studies in the field of treatment and prevention are highly recommended.

中文翻译：

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流浪猫肠道和肝脏中的螺杆菌属：分子、组织病理学和免疫组织化学研究

目的 本研究旨在确定流浪猫的十二指肠、回肠、结肠和肝脏中是否存在幽门螺杆菌属和三种幽门螺杆菌、胆汁幽门螺杆菌和犬幽门螺杆菌。此外，还进行了组织病理学和免疫组织化学分析。方法 从 30 只猫的十二指肠、回肠、结肠和肝脏中抽取样本进行分子和组织病理学评估。进行聚合酶链反应以检测上述样品中的螺杆菌属。然后，在螺杆菌阳性样品中使用物种特异性引物。结果十二指肠、回肠、结肠和肝脏样本中的螺杆菌属流行率分别为 50%、60%、50% 和 43.3%。从 18 (60%)、13 (43.3%)和 8 (26. 7%）的猫，分别。免疫组化结果证实肠隐窝或十二指肠、回肠、结肠和肝窦的粘膜层中存在细菌。结论在本研究中，十二指肠和肝脏的并发感染是明显的。此外，猫中幽门螺杆菌作为一种众所周知的人类病原体的高流行率应予以考虑。阿瓦士流浪猫的肠道和肝脏中幽门螺杆菌的高发病率是显而易见的。根据幽门螺杆菌的人畜共患病重要性，强烈建议在治疗和预防领域进行更多研究。十二指肠和肝脏并发感染明显。此外，猫中幽门螺杆菌作为一种众所周知的人类病原体的高流行率应予以考虑。阿瓦士流浪猫的肠道和肝脏中幽门螺杆菌的高发病率是显而易见的。根据幽门螺杆菌的人畜共患病重要性，强烈建议在治疗和预防领域进行更多研究。十二指肠和肝脏并发感染明显。此外，猫中幽门螺杆菌作为一种众所周知的人类病原体的高流行率应予以考虑。阿瓦士流浪猫的肠道和肝脏中幽门螺杆菌的高发病率是显而易见的。根据幽门螺杆菌的人畜共患病重要性，强烈建议在治疗和预防领域进行更多研究。