With the wide application of fullerenols in biomedicine, their environmental exposure risks and toxicity to organisms have been extensively studied. However, there is still a lack of knowledge about the distribution of fullerenols in organisms as an important aspect of their mechanism of toxicity. High-resolution matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI-IMS) is an emerging technology for researching the distribution of molecules in biological tissue samples. Using this high-resolution technique, we map the distribution of fullerenols in zebrafish tissues, and the results suggest that fullerenols enter the gill, intestine, and muscle tissues and even permeate the blood-brain barrier, reaching the brain of zebrafish after aquatic exposure. Moreover, from the MS images of fullerenols, the distribution amount of fullerenols is highest in the gill, followed by that in the intestine and the small amount in muscle and brain tissues. As an emerging environmental pollutant, the establishment of this research method will provide a new method for the study of the environmental toxicity of carbon nanomaterials. Our results also indicated that this high-resolution imaging method could be applied to explore the mechanism of interaction between carbon nanomaterials and biological systems at the cellular level in the future.

随着富勒烯醇在生物医学中的广泛应用，已经广泛研究了它们在环境中的暴露风险以及对生物的毒性。然而，仍然缺乏关于富勒烯醇在生物体中的分布作为其毒性机制的重要方面的知识。高分辨率基质辅助激光解吸/电离成像质谱（MALDI-IMS）是一种新兴的技术，用于研究生物组织样品中分子的分布。使用这种高分辨率技术，我们绘制了斑马鱼组织中富勒烯醇的分布图，结果表明，富勒烯醇进入the，肠和肌肉组织，甚至渗入血脑屏障，在水生暴露后到达斑马鱼的大脑。而且，从富勒烯醇的MS图像中，富勒烯醇的分布量在the中最高，其次在肠中，而在肌肉和脑组织中的分布较少。作为一种新兴的环境污染物，该研究方法的建立将为研究碳纳米材料的环境毒性提供新的方法。我们的结果还表明，这种高分辨率的成像方法可以在未来的细胞水平上探索碳纳米材料与生物系统之间相互作用的机理。