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Visualization of the tissue distribution of fullerenols in zebrafish (Danio rerio) using imaging mass spectrometry

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Abstract

With the wide application of fullerenols in biomedicine, their environmental exposure risks and toxicity to organisms have been extensively studied. However, there is still a lack of knowledge about the distribution of fullerenols in organisms as an important aspect of their mechanism of toxicity. High-resolution matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI-IMS) is an emerging technology for researching the distribution of molecules in biological tissue samples. Using this high-resolution technique, we map the distribution of fullerenols in zebrafish tissues, and the results suggest that fullerenols enter the gill, intestine, and muscle tissues and even permeate the blood-brain barrier, reaching the brain of zebrafish after aquatic exposure. Moreover, from the MS images of fullerenols, the distribution amount of fullerenols is highest in the gill, followed by that in the intestine and the small amount in muscle and brain tissues. As an emerging environmental pollutant, the establishment of this research method will provide a new method for the study of the environmental toxicity of carbon nanomaterials. Our results also indicated that this high-resolution imaging method could be applied to explore the mechanism of interaction between carbon nanomaterials and biological systems at the cellular level in the future.

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Funding

This study was supported by the National Natural Science Foundation of China (41977210).

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Authors and Affiliations

  1. Key Laboratory of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen, 361021, China

    Qiuyue Shi, Zixing Zhang, Changzhou Yan & Xian Zhang

  2. University of Chinese Academy of Sciences, Beijing, 100049, China

    Qiuyue Shi

  3. Global Centre for Environmental Remediation, University of Newcastle, Callaghan, NSW, 2308, Australia

    Cheng Fang

  4. Cooperative Research Centre for Contamination Assessment and Remediation of the Environment, University of Newcastle, Callaghan, NSW, 2308, Australia

    Cheng Fang

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  1. Qiuyue Shi
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  2. Cheng Fang
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Correspondence to Xian Zhang.

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All animal procedures were performed in accordance with the Guidelines for Care and Use of Laboratory Animals of Chinese Academy of Sciences University and approved by the Animal Ethics Committee of the Institute of Urban Environment, Chinese Academy of Sciences. The authors declare that they have no conflict of interest.

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Shi, Q., Fang, C., Zhang, Z. et al. Visualization of the tissue distribution of fullerenols in zebrafish (Danio rerio) using imaging mass spectrometry. Anal Bioanal Chem 412, 7649–7658 (2020). https://doi.org/10.1007/s00216-020-02902-3

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