The effect of terahertz radiation on the functional activity of bovine serum albumin was studied by electron paramagnetic resonance spectroscopy using spin trapping and spin probing techniques. The use of a pH sensitive imidazoline spin trap allowed us to record the change in the binding strength of albumin with nitric oxide. The change in the intensity and width of bands in the EPR spectrum of the spin probe under terahertz irradiation was shown using a spin probe formed directly in the aqueous solution of albumin from a precursor, dihydropyrazine dioxide, which indicates a change in the number and mobility of paramagnetic centers. The observed changes were correlated with the structural characteristics of the reaction centers of bovine serum albumin, the functional groups of the amino acids of the protein.

中文翻译：

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以牛血清白蛋白为例研究太赫兹辐射与生物聚合物相互作用的分子机制

太赫兹辐射对牛血清白蛋白功能活性的影响通过电子顺磁共振光谱使用自旋捕获和自旋探测技术进行了研究。使用 pH 敏感的咪唑啉自旋阱使我们能够记录白蛋白与一氧化氮结合强度的变化。使用在前体二氢吡嗪二氧化物的白蛋白水溶液中直接形成的自旋探针显示了在太赫兹辐射下自旋探针 EPR 光谱中谱带强度和宽度的变化，这表明数量和迁移率发生了变化的顺磁中心。观察到的变化与牛血清白蛋白反应中心的结构特征、蛋白质氨基酸的官能团有关。