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A Study on Molecular Mechanisms of Terahertz Radiation Interaction with Biopolymers Based on the Example of Bovine Serum Albumin

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Abstract

The effect of terahertz radiation on the functional activity of bovine serum albumin was studied by electron paramagnetic resonance spectroscopy using spin trapping and spin probing techniques. The use of a pH sensitive imidazoline spin trap allowed us to record the change in the binding strength of albumin with nitric oxide. The change in the intensity and width of bands in the EPR spectrum of the spin probe under terahertz irradiation was shown using a spin probe formed directly in the aqueous solution of albumin from a precursor, dihydropyrazine dioxide, which indicates a change in the number and mobility of paramagnetic centers. The observed changes were correlated with the structural characteristics of the reaction centers of bovine serum albumin, the functional groups of the amino acids of the protein.

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ACKNOWLEDGMENTS

The authors thank the Spectroscopy and Optics Center for Collective Use of the Institute of Automation and Electrometry, Siberian Branch of the Russian Academy of Sciences, for providing the source of terahertz radiation.

Funding

The studies were carried out within the state program of the Ministry of Science and Higher Education of the Russian Federation (AAAA-A17-117030310290-5).

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Authors and Affiliations

  1. Institute of Laser Physics, Siberian Branch, Russian Academy of Sciences, 630090, Novosibirsk, Russia

    E. F. Nemova, O. P. Cherkasova & N. A. Nikolaev

  2. Institute of Automation and Electrometry, Siberian Branch, Russian Academy of Sciences, 630090, Novosibirsk, Russia

    N. A. Nikolaev

  3. Voevodsky Institute of Chemical Kinetics and Combustion, Siberian Branch, Russian Academy of Sciences, 630090, Novosibirsk, Russia

    E. F. Nemova & G. G. Dultseva

Authors
  1. E. F. Nemova
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  2. O. P. Cherkasova
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  3. N. A. Nikolaev
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  4. G. G. Dultseva
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Corresponding author

Correspondence to E. F. Nemova.

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Conflict of interests. The authors declare that they have no conflict of interest.

Statement on the welfare of animals. This article does not contain any studies involving animals performed by any of the authors.

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Translated by D. Novikova

Abbreviations: BSA, bovine serum albumin; DPDO, 1,4-dihydropyrazine dioxide; EPR, electron paramagnetic resonance; PMIO, 1,2,2,5,5-pentamethyl-3-imidazoline-3-oxide.

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Nemova, E.F., Cherkasova, O.P., Nikolaev, N.A. et al. A Study on Molecular Mechanisms of Terahertz Radiation Interaction with Biopolymers Based on the Example of Bovine Serum Albumin. BIOPHYSICS 65, 410–415 (2020). https://doi.org/10.1134/S000635092003015X

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  • DOI: https://doi.org/10.1134/S000635092003015X

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