Abstract

The effect of donors of hydrogen sulfide (50 μM sodium hydrosulfide NaHS) and nitric oxide (500 μM sodium nitroprusside, SNP) on the salt resistance of wild type (Col-0) Arabidopsis plants (A-rabidopsis thaliana L. Heynh.) and those defective in jasmonate signaling—coi1 (mutant for the gene encoding protein COI1 involved in the removal of repressor proteins of transcriptional factors of jasmonate signaling) and jin1 (mutant defective in the gene encoding the transcription factor JIN1/MYC2)—have been compared. NO and H₂S donors had a similar positive effect on the salt resistance of wild-type plants, which was manifested in a decrease under their influence of water deficiency of leaves, a decrease in oxidative damage, and stabilization of membrane permeability and chlorophyll content under the influence of 175 mM NaCl. The activity of superoxide dismutase (SOD) and catalase (CAT) also increased under the influence of NaHS and SNP treatment during salinization in Col-0 plants but the stress-induced accumulation of proline decreased. Pretreatment of coi1 and jin1 mutants with NO and H₂S donors did not prevent the increase in lipid peroxidation caused by the action of NaCl and did not contribute to a decrease in membrane permeability and preservation of the pool of chlorophylls under stress conditions. In both mutants treated with NaHS or SNP, there was no increase in the activity of SOD and CAT under the action of salt. Donor treatment of NO and H₂S did not affect the magnitude of the water deficit and the content of proline in the leaves of the mutant jin1. However, the mutant coi1 presalt treatment with NaHS or SNP reduced the manifestation of water deficiency and proline accumulation. The conclusion is drawn on the involvement of jasmonate signaling components in the implementation of the stress-protective action of donors of hydrogen sulfide and nitric oxide. It is assumed that the transcription factor JIN1/MYC2 plays a more important role in the processes of salt resistance induction of Arabidopsis plants by exogenous NO and H₂S compared to protein COI1.

中文翻译：

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茉莉酸信号成分参与H 2 S和NO供体诱导的拟南芥耐盐性的发展

摘要

硫化氢（50μM氢硫化钠NaHS）和一氧化氮（500μM硝普钠，SNP）的供体对野生型（Col-0）拟南芥植物（A- rabidopsis thaliana L. Heynh。）和比较了那些在茉莉酸信号中存在缺陷的基因，即coi1（与除去茉莉酸信号转录因子的阻遏蛋白有关的编码蛋白COI1的基因的突变体）和jin1（在编码转录因子JIN1 / MYC2的基因中的突变体缺陷）。NO和H ₂S供体对野生型植物的耐盐性具有相似的积极作用，表现为在叶片缺水的影响下其降低，氧化损伤的降低，以及在水分胁迫下膜通透性和叶绿素含量的稳定。 175毫米氯化钠。在Na-0和NaCl处理下，Col-0植物在盐碱化过程中超氧化物歧化酶（SOD）和过氧化氢酶（CAT）的活性也增加，但胁迫诱导的脯氨酸积累减少。的预处理COI1和进1与NO和H突变体₂S供体不能阻止由NaCl作用引起的脂质过氧化作用的增加，并且也不能在压力条件下导致膜通透性的降低和叶绿素池的保存。在用NaHS或SNP处理的两个突变体中，在盐的作用下SOD和CAT的活性均未增加。施主处理NO和H ₂ S不会影响突变体jin1的水分亏缺程度和脯氨酸含量。但是，突变体coi1用NaHS或SNP进行盐前处理可减少缺水和脯氨酸积累的表现。结论是茉莉酸信号成分参与硫化氢和一氧化氮供体的应力保护作用的实现。假定与蛋白COI1相比，转录因子JIN1 / MYC2在外源NO和H ₂ S诱导拟南芥植物的耐盐性过程中起着更重要的作用。