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Participation of Jasmonate Signaling Components in the Development of Arabidopsis thaliana’s Salt Resistance Induced by H2S and NO Donors

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Abstract

The effect of donors of hydrogen sulfide (50 μM sodium hydrosulfide NaHS) and nitric oxide (500 μM sodium nitroprusside, SNP) on the salt resistance of wild type (Col-0) Arabidopsis plants (A-rabidopsis thaliana L. Heynh.) and those defective in jasmonate signaling—coi1 (mutant for the gene encoding protein COI1 involved in the removal of repressor proteins of transcriptional factors of jasmonate signaling) and jin1 (mutant defective in the gene encoding the transcription factor JIN1/MYC2)—have been compared. NO and H2S donors had a similar positive effect on the salt resistance of wild-type plants, which was manifested in a decrease under their influence of water deficiency of leaves, a decrease in oxidative damage, and stabilization of membrane permeability and chlorophyll content under the influence of 175 mM NaCl. The activity of superoxide dismutase (SOD) and catalase (CAT) also increased under the influence of NaHS and SNP treatment during salinization in Col-0 plants but the stress-induced accumulation of proline decreased. Pretreatment of coi1 and jin1 mutants with NO and H2S donors did not prevent the increase in lipid peroxidation caused by the action of NaCl and did not contribute to a decrease in membrane permeability and preservation of the pool of chlorophylls under stress conditions. In both mutants treated with NaHS or SNP, there was no increase in the activity of SOD and CAT under the action of salt. Donor treatment of NO and H2S did not affect the magnitude of the water deficit and the content of proline in the leaves of the mutant jin1. However, the mutant coi1 presalt treatment with NaHS or SNP reduced the manifestation of water deficiency and proline accumulation. The conclusion is drawn on the involvement of jasmonate signaling components in the implementation of the stress-protective action of donors of hydrogen sulfide and nitric oxide. It is assumed that the transcription factor JIN1/MYC2 plays a more important role in the processes of salt resistance induction of Arabidopsis plants by exogenous NO and H2S compared to protein COI1.

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Funding

This work was carried out as part of the Role of Signaling Mediators and Compounds with Hormonal Activity in the Formation of Adaptive Plant Responses to Abiotic Stressors project funded by the state budget of Ukraine (state registration no. 0117U002427).

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Authors and Affiliations

  1. Dokuchaev Kharkiv National Agrarian University, 62483, Kharkiv, Ukraine

    T. O. Yastreb, Yu. E. Kolupaev & M. A. Shkliarevskyi

  2. Institute of Cell Biology and Genetic Engineering, National Academy of Sciences of Ukraine, 02000, Kyiv, Ukraine

    A. P. Dmitriev

Authors
  1. T. O. Yastreb
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  2. Yu. E. Kolupaev
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  3. M. A. Shkliarevskyi
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  4. A. P. Dmitriev
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Correspondence to Yu. E. Kolupaev.

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This article does not contain any research involving humans and animals as research objects.

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Abbreviations: PTIO—2-phenyl-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (NO scavenger); CAT—catalase; SNP—sodium nitroprusside (NO donor); SOD—superoxide dismutase.

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Yastreb, T.O., Kolupaev, Y.E., Shkliarevskyi, M.A. et al. Participation of Jasmonate Signaling Components in the Development of Arabidopsis thaliana’s Salt Resistance Induced by H2S and NO Donors. Russ J Plant Physiol 67, 827–834 (2020). https://doi.org/10.1134/S1021443720050192

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  • DOI: https://doi.org/10.1134/S1021443720050192

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