Mulberry (Morus spp.), represents a set of plants grown in Brazil for production of fruits, leaves for feeding silkworms and ruminant animals, and for landscape. In 2017 mulberry leaves with symptoms of spots were collected in Santa Catarina (SC) state and in the Distrito Federal (DF). Three fungal isolates obtained from M. nigra in DF and one isolate obtained from M. alba in SC state were characterized by both molecular and morphological tools. Phylogenetic analysis showed that all isolates were grouped with isolates belonging to the species Neophloeospora maculans. All isolates were pathogenic on leaves of M. nigra seedlings under greenhouse conditions and were re-isolated in pure culture, completing the Koch’s postulates. One isolate was morphologically characterized, and it showed colonies with slow growth, grayish-white color and conidia hyaline, septated, filiform, straight or curved, measuring 56 (25–70) × 4.5 (2.5–5) that confirms the molecular identification. This work reposts M. nigra as a new host of N. maculans in Brazil, as well as expands the geographic distribution of this fungus in the country. This information is potentially relevant for establishing effective disease management strategies.

桑树（桑属（Morus spp。））代表了一组在巴西种植的植物，用于生产水果，用于喂养蚕和反刍动物的叶子以及用于景观。2017年，在圣卡塔琳娜州（SC）州和联邦地区（DF）中收集了有斑点症状的桑叶。从获得的三个真菌分离M.黑从获得的DF和一个分离株M.粉由两个分子和形态学工具SC状态进行表征。系统发育分析表明，所有分离株都与属于斑斑线菌的分离株分组。所有分离株在黑分枝杆菌的叶子上均具有致病性温室条件下的幼苗，并在纯培养物中重新分离，完成了科赫的假设。一种分离物经过形态学表征，显示菌落生长缓慢，灰白色和分生孢子透明，分隔，丝状，笔直或弯曲，大小为56（25-70）×4.5（2.5-5），证实了分子鉴定。这项工作将黑芒猪笼草（M. nigra）改种为巴西黄斑猪笼草的新寄主，并扩大了该真菌在巴西的地理分布。此信息可能与建立有效的疾病管理策略有关。