Amino acids are important metabolites for tissue metabolism, growth, maintenance, and repair, which are basic life necessities. Therefore, summarizing analytical methods for amino acid determination in organisms is important. In the past decades, analytical methods for amino acids have developed rapidly but have not been fully explored. Thus, this article provides reference to analytical methods for amino acids in organisms for food and human research. Present amino acid analysis methods include thin-layer chromatography, high-performance liquid chromatography, liquid chromatography–mass spectrometer, gas chromatography–mass spectrometry, capillary electrophoresis, nuclear magnetic resonance, and amino acid analyzer analysis.

氨基酸是用于组织代谢，生长，维持和修复的重要代谢物，这是基本的生活必需品。因此，总结生物中氨基酸测定的分析方法很重要。在过去的几十年中，氨基酸的分析方法发展迅速，但尚未得到充分探索。因此，本文为食品和人体研究中生物体中氨基酸的分析方法提供了参考。当前的氨基酸分析方法包括薄层色谱，高效液相色谱，液相色谱-质谱仪，气相色谱-质谱仪，毛细管电泳，核磁共振和氨基酸分析仪分析。