Abstract
Amino acids are important metabolites for tissue metabolism, growth, maintenance, and repair, which are basic life necessities. Therefore, summarizing analytical methods for amino acid determination in organisms is important. In the past decades, analytical methods for amino acids have developed rapidly but have not been fully explored. Thus, this article provides reference to analytical methods for amino acids in organisms for food and human research. Present amino acid analysis methods include thin-layer chromatography, high-performance liquid chromatography, liquid chromatography–mass spectrometer, gas chromatography–mass spectrometry, capillary electrophoresis, nuclear magnetic resonance, and amino acid analyzer analysis.
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Abbreviations
- AA:
-
Amino acid
- ACBA:
-
4-Amino-2-chlorobenzoic acid
- AQC:
-
6-Aminoquinolyl-N-hydroxysuccinimidyl carbamate
- BCAA:
-
Branched chain amino acid
- DEEMM:
-
Diethyl ethoxymethylenemalonate
- FMOC-Cl:
-
Fluorenylmethyl chloroformate
- HILIC:
-
Hydrophilic interaction liquid chromatography combined
- HSCE:
-
High-speed capillary electrophoresis
- OPA:
-
o-Phthalaldehyde
- PITC:
-
Phenylisothiocyanate
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The work was funded by the program of Shanghai Committee of Science and Technology in China (Grant number 17401902300).
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N Zhang and B Wang had the idea for the article, CC Zhong and CP Zou performed the literature search and data analysis, and WH Xu drafted the work. All authors read and approved the review.
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Xu, W., Zhong, C., Zou, C. et al. Analytical methods for amino acid determination in organisms. Amino Acids 52, 1071–1088 (2020). https://doi.org/10.1007/s00726-020-02884-7
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DOI: https://doi.org/10.1007/s00726-020-02884-7