In this study, polycaprolactone (PCL) scaffolds were fabricated via the freeze-casting method. Moreover, the surface of prepared constructs was modified using an oxygen plasma treatment technique for grafting the gelatin in order to improve physicochemical and biological properties. Field emission scanning electron microscopy (FE-SEM) micrographs demonstrated interconnect and unidirectional pore channels. At the same time, the modification procedure showed slight effects on the lamellar microstructure of pores. Fourier transforms infrared (FTIR) spectroscopy determined chemical characterization, which evaluates the effect of the surface treatment on the chemical structure of scaffolds. The wettability of the constructs was investigated by a water drop contact angle and swelling ratio tests. The results illustrated an increment in hydrophilicity after oxygen plasma modification, while the immobilization of gelatin enhanced more the potential of interaction with water molecules. Moreover, the increase in the biodegradation ratio was observed after modification. The in-vitro performance of constructs before and after gelatin immobilization was investigated bt the cell culture assay. Accordingly, modified scaffolds with gelatin support cell attachment and filopodia formation compared with the PCL ones. Besides, the biocompatibility of polymeric matrixes was proved by MTT assay. The results suggest that gelatin grafting on oxygen plasma modified PCL scaffold can be useful in wound healing.

在这项研究中，聚己内酯（PCL）支架是通过冷冻浇铸法制造的。此外，使用氧等离子体处理技术对制备的构建体的表面进行修饰以接枝明胶，以改善其理化和生物学特性。场发射扫描电子显微镜（FE-SEM）显微照片显示了互连和单向孔道。同时，改性程序对孔的层状微结构显示出轻微的影响。傅里叶变换红外（FTIR）光谱确定了化学特征，该化学特征评估了表面处理对支架化学结构的影响。通过水滴接触角和溶胀率测试研究了结构的润湿性。结果表明，氧等离子体改性后亲水性增加，而明胶的固定化增强了与水分子相互作用的潜力。此外，在改性后观察到生物降解率的增加。在细胞培养测定中研究了明胶固定之前和之后构建体的体外性能。因此，与PCL相比，具有明胶支持细胞附着和丝状伪足形成的修饰支架。此外，通过MTT法证明了聚合物基质的生物相容性。结果表明，明胶接枝在氧等离子体修饰的PCL支架上可用于伤口愈合。改性后观察到生物降解率的增加。在细胞培养测定中研究了明胶固定之前和之后构建体的体外性能。因此，与PCL相比，具有明胶支持细胞附着和丝状伪足形成的修饰支架。此外，通过MTT法证明了聚合物基质的生物相容性。结果表明，明胶接枝在氧等离子体修饰的PCL支架上可用于伤口愈合。改性后观察到生物降解率的增加。细胞培养测定法研究了明胶固定之前和之后的构建体的体外性能。因此，与PCL相比，具有明胶支持细胞附着和丝状伪足形成的修饰支架。此外，通过MTT法证明了聚合物基质的生物相容性。结果表明，明胶接枝在氧等离子体修饰的PCL支架上可用于伤口愈合。MTT法证明了聚合物基体的生物相容性。结果表明，明胶接枝在氧等离子体修饰的PCL支架上可用于伤口愈合。MTT法证明了聚合物基质的生物相容性。结果表明，明胶接枝在氧等离子体修饰的PCL支架上可用于伤口愈合。