Salinivibrio proteolyticus M318, a halophilic bacterium isolated from fermented shrimp paste, is able to produce polyhydroxyalkanoate (PHA) from different carbon sources. In this study, we report the whole-genome sequence of strain M138, which comprises 2 separated chromosomes and 2 plasmids, and the complete genome contains 3,605,935 bp with an average GC content of 49.9%. The genome of strain M318 contains 3341 genes, 98 tRNA genes, and 28 rRNA genes. The 16S rRNA gene sequence and average nucleotide identity analysis associated with morphological and biochemical tests showed that this strain has high homology to the reference strain Salinivibrio proteolyticus DSM 8285. The genes encoding key enzymes for PHA and ectoine synthesis were identified from the bacterial genome. In addition, the TeaABC transporter responsible for ectoine uptake from the environment and the operon doeABXCD responsible for the degradation of ectoine were also detected. Strain M318 was able to produce poly(3-hydroxybutyrate) [P(3HB)] from different carbon sources such as glycerol, maltose, glucose, fructose, and starch. The ability to produce ectoines at different NaCl concentrations was investigated. High ectoine content of 26.2% of cell dry weight was obtained by this strain at 18% NaCl. This report provides genetic information regarding adaptive mechanisms of strain M318 to stress conditions, as well as new knowledge to facilitate the application of this strain as a bacterial cell factory for the production of PHA and ectoine.

从发酵的虾酱中分离出的嗜盐细菌蛋白梭菌M318能够从不同碳源生产聚羟基链烷酸酯（PHA）。在这项研究中，我们报告了菌株M138的全基因组序列，它包含2条分离的染色体和2个质粒，完整的基因组包含3,605,935 bp，平均GC含量为49.9％。M318菌株的基因组包含3341个基因，98个tRNA基因和28个rRNA基因。与形态学和生化测试相关的16S rRNA基因序列和平均核苷酸同一性分析表明，该菌株与参照菌株Salinivibrio proteolyticus具有高度同源性DSM8285。从细菌基因组中鉴定出编码PHA和植物素合成关键酶的基因。此外，TeaABC转运蛋白负责环境中的植物素吸收和操纵子doeABXCD还检测到了导致果胶降解的原因。M318菌株能够从不同的碳源（如甘油，麦芽糖，葡萄糖，果糖和淀粉）生产聚（3-羟基丁酸酯）[P（3HB）]。研究了在不同的NaCl浓度下产生果胶的能力。在18％NaCl的条件下，该菌株获得了26.2％细胞干重的高菌素含量。该报告提供了有关菌株M318适应胁迫条件的遗传信息，以及促进该菌株作为细菌细胞工厂用于生产PHA和植物素的新知识。