Here, a new cell line, Ha168, was established from Helicoverpa armigera eggs and has been stably subcultured for over 30 passages in TNM-FH medium supplemented with 10% fetal bovine serum. The cell line consists of round and spindle-shaped cells and several giant cells. The round cells, with a cell diameter of 14.30 ± 2.804 μm, account for 77% of the cells. DNA amplification fingerprinting, random amplified polymorphic DNA analysis, and analysis of the mitochondrial cytochrome c oxidase subunit I gene confirmed that the Ha168 cells were derived from H. armigera. Karyotype analysis revealed the average chromosome number of Ha168 cells to be 71. Growth curves at passage 25 were determined and demonstrated that the cell population doubling time is 56.8 h. No mycoplasma contamination was detected in the cell line. Ha168 cells can be infected by recombinant baculovirus AcMNPV-EGFP, and exogenous protein expression level in this cell line is 70% of that in the Sf9 cell line.

在这里，从棉铃虫卵中建立了一个新的细胞系Ha168，并已在添加了10％胎牛血清的TNM-FH培养基中稳定传代了30多个传代。细胞系由圆形和纺锤形细胞以及几个巨细胞组成。圆形孔的直径为14.30±2.804μm，占圆形孔的77％。DNA扩增指纹图谱，随机扩增多态性DNA分析以及线粒体细胞色素C氧化酶亚基I基因的分析证实，Ha168细胞来源于棉铃虫。。核型分析表明，Ha168细胞的平均染色体数为71。在第25代时确定了生长曲线，并证明了细胞群倍增时间为56.8 h。在细胞系中未检测到支原体污染。Ha168细胞可以被重组杆状病毒AcMNPV-EGFP感染，该细胞系中外源蛋白表达水平是Sf9细胞系中的70％。