Abstract
Here, a new cell line, Ha168, was established from Helicoverpa armigera eggs and has been stably subcultured for over 30 passages in TNM-FH medium supplemented with 10% fetal bovine serum. The cell line consists of round and spindle-shaped cells and several giant cells. The round cells, with a cell diameter of 14.30 ± 2.804 μm, account for 77% of the cells. DNA amplification fingerprinting, random amplified polymorphic DNA analysis, and analysis of the mitochondrial cytochrome c oxidase subunit I gene confirmed that the Ha168 cells were derived from H. armigera. Karyotype analysis revealed the average chromosome number of Ha168 cells to be 71. Growth curves at passage 25 were determined and demonstrated that the cell population doubling time is 56.8 h. No mycoplasma contamination was detected in the cell line. Ha168 cells can be infected by recombinant baculovirus AcMNPV-EGFP, and exogenous protein expression level in this cell line is 70% of that in the Sf9 cell line.
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This work was supported by grants from the National Natural Science Foundation of China (No. 31872290 and 31572329) and the National Key R&D Program of China (grant 2019YFD1002102).
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Editor: Tetsuji Okamoto
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Zhou, L., Li, Y., Wang, H. et al. Establishment and characterization of a new embryonic cell line from Helicoverpa armigera (Hübner). In Vitro Cell.Dev.Biol.-Animal 56, 559–566 (2020). https://doi.org/10.1007/s11626-020-00473-2
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DOI: https://doi.org/10.1007/s11626-020-00473-2