Pomegranate peels (PP) and mango leaves (ML) were analysed for nutrients and tannin contents. In an in vitro test, ten diets were prepared; six contained 2, 4 and 6% of PP or ML, three diets supplemented with mixed levels of PP and ML (1 + 1%, 2 + 2% and 3 + 3%) and control diet free of them. Gas was measured after 3, 6, 12, 24, 48 and 72 h of incubation. Methane and rumen parameters were estimated. In an in vivo experiment, 40 Ossimi lambs were divided into four groups; the first was control, other groups (T1, T2 and T3) fed diets containing 6% PP, 6% ML or mix levels (3% PP + 3%ML), respectively, for 2 months. Results showed that PP and ML were rich in tannins. In the in vitro test, a maximum reduction in gas, methane and NH3-N was in 6% PP, 6% ML and mixed levels (3% PP + 3% ML). In the in vivo experiment, there were no differences in growth and digestibility of DM and nutrients between treatment groups. Only a lowered DM intake and protein digestibility in lambs fed 6% PP. Gas and methane emission was decreased significantly in lambs fed 6% PP, compared to other groups. TVFAs and NH3-N were decreased for treatment groups. Also, all treatments did not show any pathological changes in liver function or on oxidative stress markers. In conclusion, PP and ML can be used in sheep diets at inclusion levels of 6% and mixture without detrimental effects on general health of Ossimi lambs.

中文翻译：

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不同来源和水平的单宁对奥西米羔羊生活性能和抗氧化反应的影响

分析石榴皮 (PP) 和芒果叶 (ML) 的营养成分和单宁含量。在一个体外试验，准备十种​​饮食；六个含有 2%、4% 和 6% 的 PP 或 ML，三种添加混合水平的 PP 和 ML（1 + 1%、2 + 2% 和 3 + 3%）的日粮和不含它们的对照日粮。在孵育 3、6、12、24、48 和 72 小时后测量气体。估计甲烷和瘤胃参数。在一个体内实验，将40只Ossimi羔羊分成四组；第一个是对照组，其他组（T1、T2 和 T3）分别饲喂含有 6% PP、6% ML 或混合水平（3% PP + 3%ML）的日粮，持续 2 个月。结果表明，PP和ML富含单宁。在里面体外测试中，气体、甲烷和 NH3-N 的最大减少量为 6% PP、6% ML 和混合水平（3% PP + 3% ML）。在里面体内实验表明，处理组间干物质和营养物质的生长和消化率没有差异。饲喂 6% PP 的羔羊仅降低了 DM 摄入量和蛋白质消化率。与其他组相比，饲喂 6% PP 的羔羊的气体和甲烷排放量显着减少。治疗组的 TVFA 和 NH3-N 降低。此外，所有治疗均未显示肝功能或氧化应激标志物的任何病理变化。总之，PP 和 ML 可用于绵羊日粮中，其含量为 6% 和混合物，不会对 Ossimi 羔羊的整体健康产生不利影响。