Phytochemical screening of Calicotome villosa ethanolic extracts in respect of phenolic compounds (HPLC method), antioxidant activity (DPPH and β-carotene tests), determination of total phenolic and total flavonoid contents, and evaluation of cytotoxic (against Artemia salina), larvicidal (against Culex pipiens and Musca domestica) and anthelmintic activity (against Tubifex tubifex) have been performed. The flower extract exhibited higher biological activity than the stem extracts in all assays (DPPH, 0.6 mg/mL, IC50, β-carotene, 75.12 ± 0.73 %). There was good correlation between the antioxidant activity and total phenolic and total flavonoid contents. The flower extract exhibited significant cytotoxic activity (against A. salina) with 0.312 mg/mL, LC50 larvicidal activity (against Cx. pipiens) with 0.330 mg/mL, LC50 and anthelmintic activity (against T. tubifex) with 1.32 mg/mL, LC50 . HPLC analysis showed that vanillic acid was major component in the flower extract. In conclusion, C. villosa has good biological activity for further studies in agriculture, medicine and pesticide industry.

中文翻译：

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来自土耳其的绒毛花萼片的抗氧化、细胞毒性、杀幼虫和驱虫活性和植物化学筛选

Calicotome villosa 乙醇提取物的酚类化合物（HPLC 法）、抗氧化活性（DPPH 和 β-胡萝卜素测试）、总酚和总黄酮含量的测定以及细胞毒性评估（针对卤虫）、杀幼虫（针对Culex pipiens 和家蝇）和驱虫活性（针对 Tubifex tubifex）已经进行。在所有测定中，花提取物表现出比茎提取物更高的生物活性（DPPH，0.6 mg/mL，IC50，β-胡萝卜素，75.12 ± 0.73 %）。抗氧化活性与总酚和总黄酮含量之间存在良好的相关性。花提取物表现出显着的细胞毒活性（针对 A. salina），为 0.312 mg/mL，LC50 杀幼虫活性（针对 Cx. pipiens）为 0.330 mg/mL，LC50 和驱虫活性（针对 T.tubifex），LC50 为 1.32 mg/mL。HPLC分析表明香草酸是花提取物中的主要成分。综上所述，C. villosa 具有良好的生物活性，可用于农业、医药和农药工业的进一步研究。