Skip to main content
SpringerLink
Log in

Antioxidant, Cytotoxic, Larvicidal, and Anthelmintic Activity and Phytochemical Screening by HPLC of Calicotome villosa from Turkey

  • MEDICINAL PLANTS
  • Published:
Pharmaceutical Chemistry Journal Aims and scope

Phytochemical screening of Calicotome villosa ethanolic extracts in respect of phenolic compounds (HPLC method), antioxidant activity (DPPH and β-carotene tests), determination of total phenolic and total flavonoid contents, and evaluation of cytotoxic (against Artemia salina), larvicidal (against Culex pipiens and Musca domestica) and anthelmintic activity (against Tubifex tubifex) have been performed. The flower extract exhibited higher biological activity than the stem extracts in all assays (DPPH, 0.6 mg/mL, IC50, β-carotene, 75.12 ± 0.73 %). There was good correlation between the antioxidant activity and total phenolic and total flavonoid contents. The flower extract exhibited significant cytotoxic activity (against A. salina) with 0.312 mg/mL, LC50 larvicidal activity (against Cx. pipiens) with 0.330 mg/mL, LC50 and anthelmintic activity (against T. tubifex) with 1.32 mg/mL, LC50 . HPLC analysis showed that vanillic acid was major component in the flower extract. In conclusion, C. villosa has good biological activity for further studies in agriculture, medicine and pesticide industry.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  1. A. A. Makasci, R. Mammadov, O. Dusen, and H. I. Isik, J. Med. Plants Res., 4, 1637 (2010).

    Google Scholar 

  2. L. Huo, R. Lu, P. Li, et al., Grasas Aceites, 62, 149 (2011).

    Article  CAS  Google Scholar 

  3. Y. Y. Lim and J. Murtijaya, LWT, 40, 1664 – 1669 (2007).

    Article  CAS  Google Scholar 

  4. G. Zengin, A. Aktumsek, G. O. Guler, et al., Wagenitz. Rec. Nat. Prod., 5(2), 123 – 132 (2011).

    CAS  Google Scholar 

  5. E. Sikora, E. Cieslik, T. Leszczynska, et al., Food Chem., 107, 55 – 59 (2008).

    Article  CAS  Google Scholar 

  6. K. Wang, Y. Pan, H. Wang, et al, Med. Chem. Res., 19, 166 – 176 (2010).

    Article  CAS  Google Scholar 

  7. H. Cetin, I. Cinbilgel, A. Yanikoglu, and M. Gokceoglu, Phytother Res., 20, 1088 – 1090 (2006).

    Article  Google Scholar 

  8. C. Aydin and R. Mammadov, Marmara Pharm J., 21, 30 – 37 (2017).

    Article  CAS  Google Scholar 

  9. The Plant List. Version 1. Published on the Internet; http: //www.theplantlist.org / (2018) (1st January, 2018).

  10. A. Güner, S. Aslan, T. Ekim, et al., Nezahat Gökyiðit Vakfý Yayýnlarý, Ýstanbul, (2012).

  11. P. H. Davis (Ed.), Flora of Turkey and the East Aegean Islands, Edinburgh Univ. Press: Edinburgh, (3), 33 – 34 (1970).

  12. A. Elkhamlichi, A. El-Antri, H. El-Hajaji, et al., Arab. J. Chem., 10(2), 3580 – 3583 (2017).

    Article  Google Scholar 

  13. G. Loy, F. Cottiglia, D. Garau, et al., Farmaco, 56, 433 – 436 (2001).

    Article  CAS  Google Scholar 

  14. M. A. Dessí, M. Deiana, A. Rosa, et al., Phytother. Res., 15, 511 – 518 (2001).

    Article  Google Scholar 

  15. A. El-Antri, I. Messouri, R. C. Tlemçani, et al., Molecules, 9, 568 – 573 (2004).

    Article  CAS  Google Scholar 

  16. L. Pistelli, C. Fiumi, I. Morelli, and I. Giachi, Fitoterapia, 74, 417 – 419 (2003).

    Article  CAS  Google Scholar 

  17. M. Turan and R. Mammadov, Pharmacol. Pharm., 9, 100 – 116 (2018).

    Article  CAS  Google Scholar 

  18. C. Wu, F. Chen, X. Wang, et al., Food Chem., 96, 220 – 227 (2006).

    Article  CAS  Google Scholar 

  19. I. Amin, M. M. Zamaliah, W. F. Chin, Food Chem., 87, 581 – 586 (2004).

    Article  Google Scholar 

  20. K. Slinkard, L. Vernon, and V. L. Singleton, Am. J. Enol. Viticult., 28, 49 – 55 (1977).

    CAS  Google Scholar 

  21. A. Arvouet-Grand, B. Vennat, A. Pourrat, and P. Legret, J. Pharm. Belg., 49, 462 – 468 (1994).

    CAS  PubMed  Google Scholar 

  22. F. Caponio, V. Alloggio, and T. Gomesb, Food Chem., 64, 203 – 209 (1999).

    Article  CAS  Google Scholar 

  23. A. V. Krishnaraju, T. V. N. Rao, D. Sundararaju, et al., Int. J. Appl. Sci. Eng. Res., 3, 125 – 134 (2005).

    Google Scholar 

  24. H. Çetin, F. Erler, and A. Yanikoglu, J. Insect Sci., 6, 1 – 4 (2006).

    Article  Google Scholar 

  25. E. O. Ajaiyeoba, P. A. Onocha, and O. T. Olarenqaju, Pharm. Biol., 39, 217 – 220 (2001).

    Article  Google Scholar 

  26. G. Du, M. Li, F. Ma, and D. Liang, Food Chem., 113, 557 – 562 (2009).

    Article  CAS  Google Scholar 

  27. A. Elkhamlichi, H. El-Hajaji, H. Faraj, et al., J. Appl. Pharm. Sci., 7, 192 – 198 (2017).

    CAS  Google Scholar 

  28. R. Cherfia, M. Kara-Ali, I. Talhi, et al., J. Porphyrins Phthalocyanines, 9, 185 – 196 (2017).

    CAS  Google Scholar 

  29. A. Guaadaoui, I. El-Alami, M. Abid, et al., Int. J. Green Herbal Chem., 5, 93 – 111 (2016).

    CAS  Google Scholar 

  30. M. Govindarajan and R. Sivakumar, Parasitol. Res., 113, 777 – 791 (2014).

    Article  Google Scholar 

  31. C. Aydin and R. Mammadov, Int. J. Food Properties, 20, 2276 – 2285 (2017).

    Article  CAS  Google Scholar 

  32. M. H. Hossain, M. S. H. Kabir, T. A. Chowdhury, et al., Brit. J. Pharm. Res., 8, 1 – 7 (2015).

    CAS  Google Scholar 

  33. J. Alhage, H. Elbitar, S. Taha, et al., Molecules, 23, 850 – 870 (2018).

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biology, Faculty of Art & Science, Pamukkale University, Denizli, Turkey

    Murat Turan

  2. Department of Molecular Biology and Genetics, Faculty of Science, Muğla Sıtkı Koçman University, Muğla, Turkey

    Ramazan Mammadov

Authors
  1. Murat Turan
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Ramazan Mammadov
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Ramazan Mammadov.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Turan, M., Mammadov, R. Antioxidant, Cytotoxic, Larvicidal, and Anthelmintic Activity and Phytochemical Screening by HPLC of Calicotome villosa from Turkey. Pharm Chem J 54, 478–483 (2020). https://doi.org/10.1007/s11094-020-02225-8

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11094-020-02225-8

Keywords

Search

Navigation