Production of biofuel by microalgae can become economically viable if other high-value commercial products such as carotenoid are simultaneously produced. This study aimed at enhancement of lipid and carotenoid productivity by an oleaginous microalga sp. Desmodesmus subspicatus LC172266 by supplementing Nitrogen sources to BG 11 medium under batch or fed batch mixotrophic condition. Optimum urea supplement (0.2 gL−1) to BG 11 medium enhanced carotenoid content of the D. subspicatus by about 3.7 fold (3.02±0.002 mgg−1 cell) and 3.0 fold (3.86±0.001 mgg−1 cell) using glucose or glycerol as organic carbon sources, respectively. Also, lipid productivity was enhanced by about 2.0 (0.07817±0.0028 gL−1day−1) (0.265±0.006 gg−1 cell) and 2.5 (0.08426±0.0008 gL−1day−1) (0.285±0.02 gg−1 cell) fold. Fed-batch cultivation with glucose at a feed rate of 0.5 gL−1 per day enhanced both carotenoid and lipid productivities of D. subspicatus by 34% and 45%, respectively, after eight days of mixotrophic cultivation.

中文翻译：

---

混合营养条件下通过营养补充策略在淡水绿色微藻 Desmodesmus subspicatus LC172266 中脂质和类胡萝卜素的同时积累

如果同时生产其他高价值商业产品（如类胡萝卜素），通过微藻生产生物燃料在经济上是可行的。本研究旨在提高产油微藻的脂质和类胡萝卜素生产力。Desmodesmus subspicatus LC172266 通过在分批或分批补料混养条件下向 BG 11 培养基补充氮源。使用葡萄糖或甘油，向 BG 11 培养基中添加最佳尿素 (0.2 gL-1) 可将 D. subspicatus 的类胡萝卜素含量提高约 3.7 倍（3.02±0.002 mg-1 细胞）和 3.0 倍（3.86±0.001 mg-1 细胞）分别作为有机碳源。此外，脂质生产力提高了约 2.0 (0.07817±0.0028 gL-1day-1) (0.265±0.006 gg-1 细胞) 和 2.5 (0.08426±0.0008 gL-1day-1) (0.285±0.02 倍 gg-1 细胞) . 用葡萄糖以 0 的进料速率进行补料分批培养。