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Simultaneous accumulation of lipid and carotenoid in freshwater green microalgae Desmodesmus subspicatus LC172266 by nutrient replete strategy under mixotrophic condition

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Abstract

Production of biofuel by microalgae can become economically viable if other high-value commercial products such as carotenoid are simultaneously produced. This study aimed at enhancement of lipid and carotenoid productivity by an oleaginous microalga sp. Desmodesmus subspicatus LC172266 by supplementing Nitrogen sources to BG 11 medium under batch or fed batch mixotrophic condition. Optimum urea supplement (0.2 gL−1) to BG 11 medium enhanced carotenoid content of the D. subspicatus by about 3.7 fold (3.02±0.002 mgg−1 cell) and 3.0 fold (3.86±0.001 mgg−1 cell) using glucose or glycerol as organic carbon sources, respectively. Also, lipid productivity was enhanced by about 2.0 (0.07817±0.0028 gL−1day−1) (0.265±0.006 gg−1 cell) and 2.5 (0.08426±0.0008 gL−1day−1) (0.285±0.02 gg−1 cell) fold. Fed-batch cultivation with glucose at a feed rate of 0.5 gL−1 per day enhanced both carotenoid and lipid productivities of D. subspicatus by 34% and 45%, respectively, after eight days of mixotrophic cultivation.

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Author notes

  1. Chijioke Nwoye Eze

    Present address: Current addresses: Department of Science Laboratory Technology, University of Nigeria, Nsukka, Nigeria

Authors and Affiliations

  1. Department of Microbiology, Faculty of Biological Sciences, University of Nigeria, Nsukka, Nigeria

    Chijioke Nwoye Eze & James Chukwuma Ogbonna

  2. Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Japan

    Chijioke Nwoye Eze & Hideki Aoyagi

Authors
  1. Chijioke Nwoye Eze
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  2. Hideki Aoyagi
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  3. James Chukwuma Ogbonna
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Correspondence to Chijioke Nwoye Eze.

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Eze, C.N., Aoyagi, H. & Ogbonna, J.C. Simultaneous accumulation of lipid and carotenoid in freshwater green microalgae Desmodesmus subspicatus LC172266 by nutrient replete strategy under mixotrophic condition. Korean J. Chem. Eng. 37, 1522–1529 (2020). https://doi.org/10.1007/s11814-020-0564-8

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  • DOI: https://doi.org/10.1007/s11814-020-0564-8

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