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In vivo monitoring of carbonic anhydrase expression during the growth of larval zebrafish: a new environment-sensitive fluorophore for responsive turn-on fluorescence.
Chemical Communications ( IF 4.3 ) Pub Date : 2020-08-19 , DOI: 10.1039/d0cc03090b
Chih-Hung Chou,Chang-Yi Wu,Chun-Lin Chen,Jun-Qing Zhou,Yu-Chen Kao,Hsing-Yin Chen,Po-Chiao Lin

This study monitors the dynamic progress of a newly developed background-free, target responsive strategy; 2,3-dihydroquinolin-4-imine (DQI) that can instantly respond to environmental changes with fluorescence enhancement, revealing a comprehensive platform for in vivo fluorescence bioimaging of mebrane-bound carbonic anhydrase II in HeLa cells and its expression during the growth of larval zebrafish.

中文翻译:

幼虫斑马鱼生长过程中碳酸酐酶表达的体内监测:一种新的对环境敏感的荧光团,用于响应性开启荧光。

这项研究监测了新开发的无背景,目标响应策略的动态进展;2,3-二氢喹啉-4-亚胺(DQI)可以通过荧光增强立即响应环境变化,为膜结合碳酸酐酶II在HeLa细胞中的体内荧光生物成像及其在幼虫生长期间的表达提供了一个全面的平台斑马鱼。
更新日期:2020-09-24
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