The amounts of uric acid (UA) in non-invasive biological samples, such as saliva, are critical for diagnosis and therapy of gout, hyperuricemia, Lesch–Nyhan syndrome, and several other diseases. Here, disposable UA biosensors were fabricated with the screen printing technique on the substrate of flexible PET. The working electrode was modified with carbon nanotubes followed by uricase for UA detection with excellent selectivity. The biosensor showed good electrocatalytic activity toward UA with high sensitivity, low detection limit, and wide linear range, which covers the full range of UA levels in human saliva. We demonstrate that UA can be directly detected in human saliva with the biosensor and the experimental data were consistent with the clinical analysis. This study indicated that the non-invasive biosensor is an attractive and possible approach for the monitoring of salivary UA.

非侵入性生物样品（例如唾液）中的尿酸（UA）含量对于痛风，高尿酸血症，Lesch–Nyhan综合征和其他几种疾病的诊断和治疗至关重要。在这里，用丝网印刷技术在柔性PET的基底上制造了一次性UA生物传感器。用碳纳米管修饰工作电极，然后用尿酸酶以优异的选择性对UA检测。该生物传感器对UA表现出良好的电催化活性，具有高灵敏度，低检测限和宽线性范围，涵盖了人类唾液中UA水平的全部范围。我们证明了可以通过生物传感器直接在人唾液中检测到UA，并且实验数据与临床分析一致。