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An electrochemical biosensor based on multi-wall carbon nanotube–modified screen-printed electrode immobilized by uricase for the detection of salivary uric acid

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Abstract

The amounts of uric acid (UA) in non-invasive biological samples, such as saliva, are critical for diagnosis and therapy of gout, hyperuricemia, Lesch–Nyhan syndrome, and several other diseases. Here, disposable UA biosensors were fabricated with the screen printing technique on the substrate of flexible PET. The working electrode was modified with carbon nanotubes followed by uricase for UA detection with excellent selectivity. The biosensor showed good electrocatalytic activity toward UA with high sensitivity, low detection limit, and wide linear range, which covers the full range of UA levels in human saliva. We demonstrate that UA can be directly detected in human saliva with the biosensor and the experimental data were consistent with the clinical analysis. This study indicated that the non-invasive biosensor is an attractive and possible approach for the monitoring of salivary UA.

A disposable uric acid biosensor modified with carbon nanotubes followed by uricase was fabricated on flexible PET and applied for the monitoring of salivary uric acid in human saliva

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Funding

This work was financially supported by the National Natural Science Foundation of China (31741051, 21874077), Key R&D Projects of Jiangsu Province (BE 2019690), the Basic Science Research Program of Nantong City (JC2018066), the Science and Technology of R&D fund of Nantong City (XG202008-02), Qing Lan Project of Jiangsu Province, and the Jiangsu Overseas Visiting Scholar Program for University Prominent Young and Middle-Aged Teachers and Presidents.

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Authors and Affiliations

  1. School of Public Health, Nantong University, Nantong, 226019, Jiangsu, China

    Weishan Shi, Jing Li, Jie Wu, Qianying Wei, Cuili Chen, Ning Bao, Chunmei Yu & Haiying Gu

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  1. Weishan Shi
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  2. Jing Li
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  3. Jie Wu
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  7. Chunmei Yu
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Correspondence to Chunmei Yu or Haiying Gu.

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Research involving human participants and/or animals

All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards. The collection of samples was approved by the Ethics Committee of the Affiliated Hospital of Nantong University, and the experiments on human biologic samples were approved by the Ethics Committee of Nantong University.

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Shi, W., Li, J., Wu, J. et al. An electrochemical biosensor based on multi-wall carbon nanotube–modified screen-printed electrode immobilized by uricase for the detection of salivary uric acid. Anal Bioanal Chem 412, 7275–7283 (2020). https://doi.org/10.1007/s00216-020-02860-w

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  • DOI: https://doi.org/10.1007/s00216-020-02860-w

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