Previous studies showed that miR-874 expression was abnormally expressed in many tumors. However, the potential role of miR-874 in glioma remains a mystery. This study aimed to investigate its expression, clinical significance, and cellular function in glioma. A total of 105 glioma patients were enrolled in the present study. The RT-qPCR analysis was used to detect the expression of miR-874 in glioma tissues and cells. The χ² test was used to analyze the association between miR-874 expression and clinical characteristics of patients. Kaplan–Meier method and multivariate Cox regression assays were used to analyze the prognostic value of miR-874 in glioma. Cell counting kit-8 and Transwell assays were used to explore the alterations in a series of cancer-related phenotypes affected by miR-874, including cell proliferation, migration, and invasion capacities. The expression of miR-874 was significantly downregulated in human glioma tissue specimens and cell lines. Furthermore, the expression of miR-874 was associated with tumor size, KPS, and WHO grade. The decreased expression of miR-874 was associated with shorter overall survival. Then functional assays indicated that upregulation of miR-874 suppressed proliferation, migration, and invasion of glioma cells in vitro. The present study indicated that miR-874 inhibited cell proliferation, migration, and invasion of glioma cells and might be a novel prognostic biomarker and potential therapeutic target for glioma.

先前的研究表明，miR-874 表达在许多肿瘤中异常表达。然而，miR-874 在胶质瘤中的潜在作用仍然是一个谜。本研究旨在探讨其在胶质瘤中的表达、临床意义和细胞功能。本研究共招募了 105 名神经胶质瘤患者。RT-qPCR分析用于检测胶质瘤组织和细胞中miR-874的表达。χ ² _采用检验分析miR-874表达与患者临床特征的相关性。Kaplan-Meier 方法和多变量 Cox 回归分析用于分析 miR-874 在胶质瘤中的预后价值。细胞计数 kit-8 和 Transwell 检测用于探索受 miR-874 影响的一系列癌症相关表型的改变，包括细胞增殖、迁移和侵袭能力。miR-874 的表达在人胶质瘤组织标本和细胞系中显着下调。此外，miR-874 的表达与肿瘤大小、KPS 和 WHO 分级相关。miR-874 的表达降低与较短的总生存期相关。然后功能测定表明，miR-874 的上调抑制了体外胶质瘤细胞的增殖、迁移和侵袭。