Abstract
Previous studies showed that miR-874 expression was abnormally expressed in many tumors. However, the potential role of miR-874 in glioma remains a mystery. This study aimed to investigate its expression, clinical significance, and cellular function in glioma. A total of 105 glioma patients were enrolled in the present study. The RT-qPCR analysis was used to detect the expression of miR-874 in glioma tissues and cells. The χ2 test was used to analyze the association between miR-874 expression and clinical characteristics of patients. Kaplan–Meier method and multivariate Cox regression assays were used to analyze the prognostic value of miR-874 in glioma. Cell counting kit-8 and Transwell assays were used to explore the alterations in a series of cancer-related phenotypes affected by miR-874, including cell proliferation, migration, and invasion capacities. The expression of miR-874 was significantly downregulated in human glioma tissue specimens and cell lines. Furthermore, the expression of miR-874 was associated with tumor size, KPS, and WHO grade. The decreased expression of miR-874 was associated with shorter overall survival. Then functional assays indicated that upregulation of miR-874 suppressed proliferation, migration, and invasion of glioma cells in vitro. The present study indicated that miR-874 inhibited cell proliferation, migration, and invasion of glioma cells and might be a novel prognostic biomarker and potential therapeutic target for glioma.
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Data Availability
The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.
Abbreviations
- GBM:
-
Glioblastoma
- miRNAs:
-
MicroRNAs
- KPS:
-
Karnofsky Performance Scale
- NC:
-
Negative control
- CCK-8:
-
Cell counting kit-8
- CDK9:
-
Cyclin-dependent kinase 9
- HDAC1:
-
Histone deacetylase 1
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All authors conceived and designed the study and prepared the manuscript. YL, XC, WX, and JL performed the experiment, and analysed the data. YL wrote the manuscript, and LW revised it. All authors read and approved the final version.
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The present study was approved by the Research Ethics Committee of The First Affiliated Hospital of Zunyi Medical University.
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Li, Y., Chen, X., Xue, W. et al. MiR-874 Inhibits Cell Proliferation, Migration, and Invasion of Glioma Cells and Correlates with Prognosis of Glioma Patients. Neuromol Med 23, 247–255 (2021). https://doi.org/10.1007/s12017-020-08608-0
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DOI: https://doi.org/10.1007/s12017-020-08608-0