The Egyptian cotton leaf worm, Spodoptera littoralis (Boisd.), is a major agricultural lepidopterous pest causing extensive damage in a variety of crops including vegetable, cotton, fodder, and fiber crops. Heat shock protein (HSP) family members play important roles in protecting insects against environmental stressors. In this study, we characterized three putative heat shock proteins (SpliHsp70, SpliHsp90, and SpliHSF) from S. littoralis and analyzed their expression levels in response to heat, cold, ultraviolet irradiation, Bacillus thuringiensis, and Spodoptera littoralis nucleopolyhedrovirus treatments. Significant upregulation of SpliHsp70 was observed in female pupae, while the highest expression levels of SpliHsp90 and SpliHSF were found in female adults. Heat shock triggered increases in SpliHsp levels compared to cold treatment. SpliHsp90 exhibited the highest expression levels during the first 30 min of UV treatment. Both bacterial and viral pathogenic agents effected the regulation of Hsps in S. littoralis. These findings suggest that SpliHsp genes might play significant roles in the response to biotic and abiotic stress, as well as in the regulation of developmental stages.

埃及棉叶蠕虫Spodoptera littoralis (Boisd.) 是一种主要的农业鳞翅目害虫，对包括蔬菜、棉花、饲料和纤维作物在内的多种作物造成广泛损害。热休克蛋白 (HSP) 家族成员在保护昆虫免受环境压力方面发挥重要作用。在这项研究中，我们表征了来自S 的三种假定的热休克蛋白（SpliHsp70、SpliHsp90 和 SpliHSF）。littoralis并分析了它们响应热、冷、紫外线照射、苏云金芽孢杆菌和滨海斜纹夜蛾核多角体病毒处理的表达水平。SpliHsp70 的显着上调在雌性蛹中观察到，而在雌性成虫中发现SpliHsp90和SpliHSF的最高表达水平。与冷处理相比，热休克引发了SpliHsp水平的增加。SpliHsp90在紫外线处理的前 30 分钟内表现出最高的表达水平。细菌和病毒病原体均影响S中 Hsps 的调节。滨海。这些发现表明SpliHsp基因可能在对生物和非生物胁迫的响应以及发育阶段的调节中发挥重要作用。