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Molecular characterization and expression patterns of heat shock proteins in Spodoptera littoralis, heat shock or immune response?

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Cell Stress and Chaperones Aims and scope

Abstract

The Egyptian cotton leaf worm, Spodoptera littoralis (Boisd.), is a major agricultural lepidopterous pest causing extensive damage in a variety of crops including vegetable, cotton, fodder, and fiber crops. Heat shock protein (HSP) family members play important roles in protecting insects against environmental stressors. In this study, we characterized three putative heat shock proteins (SpliHsp70, SpliHsp90, and SpliHSF) from S. littoralis and analyzed their expression levels in response to heat, cold, ultraviolet irradiation, Bacillus thuringiensis, and Spodoptera littoralis nucleopolyhedrovirus treatments. Significant upregulation of SpliHsp70 was observed in female pupae, while the highest expression levels of SpliHsp90 and SpliHSF were found in female adults. Heat shock triggered increases in SpliHsp levels compared to cold treatment. SpliHsp90 exhibited the highest expression levels during the first 30 min of UV treatment. Both bacterial and viral pathogenic agents effected the regulation of Hsps in S. littoralis. These findings suggest that SpliHsp genes might play significant roles in the response to biotic and abiotic stress, as well as in the regulation of developmental stages.

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Authors and Affiliations

  1. Department of Plant Protection, Molecular Entomology Laboratory, Faculty of Agriculture, Ankara University, Diskapi, Ankara, Turkey

    Nurper Guz

  2. Department of Molecular Biology and Genetics, Necmettin Erbakan University, Meram, Konya, Turkey

    Asli Dageri

  3. Institute of Crop Science and Resource Conservation (INRES-Phytomedicine), Rheinische Friedrich-Wilhelms-University of Bonn, Bonn, Germany

    Boran Altincicek

  4. Department of Epidemiology of Microbial Diseases, Yale School of Public Health, New Haven, CT, USA

    Serap Aksoy

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  1. Nurper Guz
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Conceptualization: [Nurper Guz, Asli Dageri]; Methodology: [Nurper Guz, Asli Dageri, Boran Altincicek]; Formal analysis and investigation: [Nurper Guz, Asli Dageri, Boran Altincicek]; Writing—original draft preparation: [Nurper Guz, Asli Dageri, Boran Altincicek, Serap Aksoy]; Writing—review and editing: [Nurper Guz, Asli Dageri, Boran Altincicek, Serap Aksoy]; Funding acquisition: [Nurper Guz]; Resources: [Nurper Guz]; Supervision: [Boran Altincicek, Serap Aksoy]; Data curation: [Nurper Guz, Asli Dageri, Boran Altincicek, Serap Aksoy]; Project Administration: [Nurper Guz]; Software: [Nurper Guz, Asli Dageri]; Validation: [Nurper Guz, Asli Dageri, Boran Altincicek, Serap Aksoy]; Visualization: [Nurper Guz, Asli Dageri].

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Guz, N., Dageri, A., Altincicek, B. et al. Molecular characterization and expression patterns of heat shock proteins in Spodoptera littoralis, heat shock or immune response?. Cell Stress and Chaperones 26, 29–40 (2021). https://doi.org/10.1007/s12192-020-01149-2

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