Pectin methylesterase (PME) is a type of protein encoded by a multi-gene family and participates in seed germination, fruit ripening and stress response. To further study the function of the PME gene family in potato, this paper screened and identified potato PME gene family by bioinformatics methods, and analysed the structure, physicochemical properties, conserved domains and evolutionary relationships of the gene sequences. The results showed that the identified 54 potato PME genes can be divided into Group I (only PME domain) and Group II (both PME domain and PMEI domain). The phylogenetic tree constructed by the maximum likelihood method indicated that potato PME genes consisted of five subfamilies. The protein sequences of potato PME genes were compared with clustalx2.1 software. Most of these sequences were identified to have five conserved structural regions including Gx Yx E (Region I), QAVAxR (Region II), QDTL (Region III), GxxDFIFG (Region IV) and YLGRxWx (Region V). Conserved motifs of potato PME amino acid sequences were analysed by MEME online software. Motif1 contains three of the five conserved structural regions and motif2 and motif8 each contain one, indicating that these sequences are more conservative. The structure of 54 potato PME gene sequences was analysed by the GSDS website. It was found that there were only two parts, exon or coding sequence (CDS) between untranslated regions (UTRs), without intron structures in these sequences, indicating that the structure is relatively simple. It will provide a reference for the study on the function of potato PME gene.

果胶甲酯酶（PME）是一种由多基因家族编码的蛋白质，参与种子发芽，果实成熟和胁迫反应。为了进一步研究马铃薯PME基因家族的功能，通过生物信息学方法筛选和鉴定了马铃薯PME基因家族，并分析了该基因序列的结构，理化性质，保守域和进化关系。结果表明，鉴定出的54个马铃薯PME基因可以分为I组（仅PME结构域）和II组（PME结构域和PMEI结构域）。通过最大似然法构建的系统进化树表明，马铃薯PME基因由五个亚科组成。将马铃薯PME基因的蛋白质序列与clustalx2.1软件进行比较。这些序列中的大多数被鉴定为具有五个保守的结构区域，包括Gx Yx E（区域I），QAVAxR（区域II），QDTL（区域III），GxxDFIFG（区域IV）和YLGRxWx（区域V）。通过MEME在线软件分析了马铃薯PME氨基酸序列的保守基序。Motif1包含五个保守结构区域中的三个，motif2和motif8各自包含一个，表明这些序列更为保守。通过GSDS网站分析了54个马铃薯PME基因序列的结构。发现在非翻译区（UTR）之间只有两个部分，外显子或编码序列（CDS），在这些序列中没有内含子结构，表明该结构相对简单。为马铃薯PME基因功能的研究提供参考。