Magnetophoretic separation has gained much attention in recent years due to its easy application and low-cost fabrication compared to other active particle separation techniques. Due to the different properties of white blood cells (WBCs) and red blood cells (RBCs), it is possible to manipulate and separate them using a magnetic field. In this paper, a simple microfluidic device is proposed to fractionate WBCs and RBCs from whole blood using magnetophoretic force applied by Halbach array of three permanent magnets. Plasma streams containing WBCs and RBCs enter a simple microchip fabricated by PDMS. Permanent magnets apply positive and negative magnetophoretic forces to the RBCs and WBCs, respectively. Two cladding streams containing blood plasma are used to concentrate the cells in the magnetophoretic area. A wide range of inlet velocities and different distances of magnets from the channel (d) are investigated. It is demonstrated that the volume flow rate of core, and cladding streams, total flow rate and the distance between magnets and microchannel affect the separation efficiency individually. The results reveal that d = 0.1, 0.2, 0.3, 0.4, and 0.5 mm may lead to complete separation when core and cladding flow rates are 1 and 7 μl/h, respectively.

中文翻译：

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使用 Halbach 磁体阵列磁泳分离血细胞

与其他活性粒子分离技术相比，磁泳分离因其易于应用和低成本制造而在近年来受到广泛关注。由于白细胞 (WBC) 和红细胞 (RBC) 的不同特性，可以使用磁场来操纵和分离它们。在本文中，提出了一种简单的微流体装置，使用由三个永磁体的 Halbach 阵列施加的磁泳力从全血中分离 WBC 和 RBC。含有 WBC 和 RBC 的血浆流进入一个由 PDMS 制造的简单微芯片。永磁体分别对 RBC 和 WBC 施加正负磁泳力。两个包含血浆的包层流用于将细胞集中在磁泳区。研究了大范围的入口速度和磁体与通道 (d) 的不同距离。结果表明，纤芯和包层流的体积流量、总流量以及磁体与微通道之间的距离分别影响分离效率。结果表明，当纤芯和包层流速分别为 1 和 7 μl/h 时，d = 0.1、0.2、0.3、0.4 和 0.5 mm 可能导致完全分离。