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On magnetophoretic separation of blood cells using Halbach array of magnets

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Abstract

Magnetophoretic separation has gained much attention in recent years due to its easy application and low-cost fabrication compared to other active particle separation techniques. Due to the different properties of white blood cells (WBCs) and red blood cells (RBCs), it is possible to manipulate and separate them using a magnetic field. In this paper, a simple microfluidic device is proposed to fractionate WBCs and RBCs from whole blood using magnetophoretic force applied by Halbach array of three permanent magnets. Plasma streams containing WBCs and RBCs enter a simple microchip fabricated by PDMS. Permanent magnets apply positive and negative magnetophoretic forces to the RBCs and WBCs, respectively. Two cladding streams containing blood plasma are used to concentrate the cells in the magnetophoretic area. A wide range of inlet velocities and different distances of magnets from the channel (d) are investigated. It is demonstrated that the volume flow rate of core, and cladding streams, total flow rate and the distance between magnets and microchannel affect the separation efficiency individually. The results reveal that d = 0.1, 0.2, 0.3, 0.4, and 0.5 mm may lead to complete separation when core and cladding flow rates are 1 and 7 μl/h, respectively.

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References

  1. Dalili A, Samiei E, Hoorfar M (2019) A review of sorting, separation and isolation of cells and microbeads for biomedical applications: microfluidic approaches. Analyst 144(1):87–113

    Google Scholar 

  2. Sajeesh P, Sen AK (2014) Particle separation and sorting in microfluidic devices: a review. Microfluid Nanofluid 17(1):1–52

    Google Scholar 

  3. Yan S, Tan SH, Li Y, Tang S, Teo AJ, Zhang J, Zhao Q, Yuan D, Sluyter R, Nguyen N-T (2018) A portable, hand-powered microfluidic device for sorting of biological particles. Microfluid Nanofluid 22(1):8

    Google Scholar 

  4. Chen J, Chen D, Yuan T, Chen X, Xie Y, Fu H, Cui D, Fan X, Oo MKK (2014) Blood plasma separation microfluidic chip with gradual filtration. Microelectron Eng 128:36–41

    Google Scholar 

  5. Kang Y-T, Doh I, Byun J, Chang HJ, Cho Y-H (2017) Label-free rapid viable enrichment of circulating tumor cell by photosensitive polymer-based microfilter device. Theranostics 7(13):3179

    Google Scholar 

  6. Liu C, Mauk M, Gross R, Bushman FD, Edelstein PH, Collman RG, Bau HH (2013) Membrane-based, sedimentation-assisted plasma separator for point-of-care applications. Anal Chem 85(21):10463–10470

    Google Scholar 

  7. Amini H, Lee W, Di Carlo D (2014) Inertial microfluidic physics. Lab Chip 14(15):2739–2761

    Google Scholar 

  8. Di Carlo D, Irimia D, Tompkins RG, Toner M (2007) Continuous inertial focusing, ordering, and separation of particles in microchannels. Proc Natl Acad Sci 104(48):18892–18897

    Google Scholar 

  9. Martel JM, Toner M (2012) Inertial focusing dynamics in spiral microchannels. Phys Fluids 24(3):032001

    Google Scholar 

  10. Rafeie M, Zhang J, Asadnia M, Li W, Warkiani ME (2016) Multiplexing slanted spiral microchannels for ultra-fast blood plasma separation. Lab Chip 16(15):2791–2802

    Google Scholar 

  11. Dincau BM, Aghilinejad A, Hammersley T, Chen X, Kim J-H (2018) Deterministic lateral displacement (DLD) in the high Reynolds number regime: high-throughput and dynamic separation characteristics. Microfluid Nanofluid 22(6):59

    Google Scholar 

  12. Holm SH, Beech JP, Barrett MP, Tegenfeldt JO (2011) Separation of parasites from human blood using deterministic lateral displacement. Lab Chip 11(7):1326–1332

    Google Scholar 

  13. Huang LR, Cox EC, Austin RH, Sturm JC (2004) Continuous particle separation through deterministic lateral displacement. Science 304(5673):987–990

    Google Scholar 

  14. Loutherback K, D’Silva J, Liu L, Wu A, Austin RH, Sturm JC (2012) Deterministic separation of cancer cells from blood at 10 mL/min. AIP Adv 2(4):042107

    Google Scholar 

  15. Jain A, Posner JD (2008) Particle dispersion and separation resolution of pinched flow fractionation. Anal Chem 80(5):1641–1648

    Google Scholar 

  16. Vig AL, Kristensen A (2008) Separation enhancement in pinched flow fractionation. Appl Phys Lett 93(20):203507

    Google Scholar 

  17. Yamada M, Nakashima M, Seki M (2004) Pinched flow fractionation: continuous size separation of particles utilizing a laminar flow profile in a pinched microchannel. Anal Chem 76(18):5465–5471

    Google Scholar 

  18. Pethig R (2010) Dielectrophoresis: status of the theory, technology, and applications. Biomicrofluidics 4(2):022811

    Google Scholar 

  19. Shafiee H, Caldwell JL, Sano MB, Davalos RV (2009) Contactless dielectrophoresis: a new technique for cell manipulation. Biomed Microdev 11(5):997

    Google Scholar 

  20. Zhu H, Lin X, Su Y, Dong H, Wu J (2015) Bioelectronics. Screen-printed microfluidic dielectrophoresis chip for cell separation. Biosens Bioelectron 63:371–378

    Google Scholar 

  21. Das D, Biswas K, Das S (2014) Physics. A microfluidic device for continuous manipulation of biological cells using dielectrophoresis. Med Eng Phys 36(6):726–731

    Google Scholar 

  22. Vykoukal J, Vykoukal DM, Freyberg S, Alt EU, Gascoyne PR (2008) Enrichment of putative stem cells from adipose tissue using dielectrophoretic field-flow fractionation. Lab Chip 8(8):1386–1393

    Google Scholar 

  23. Yunus NAM, Nili H, Green NG (2013) Continuous separation of colloidal particles using dielectrophoresis. Electrophoresis 34(7):969–978

    Google Scholar 

  24. Ding X, Li P, Lin S-CS, Stratton ZS, Nama N, Guo F, Slotcavage D, Mao X, Shi J, Costanzo F (2013) Surface acoustic wave microfluidics. Lab Chip 13(18):3626–3649

    Google Scholar 

  25. Petersson F, Åberg L, Swärd-Nilsson A-M, Laurell T (2007) Free flow acoustophoresis: microfluidic-based mode of particle and cell separation. Anal Chem 79(14):5117–5123

    Google Scholar 

  26. Shi J, Huang H, Stratton Z, Huang Y, Huang T (2009) Continuous particle separation in a microfluidic channel via standing surface acoustic waves (SSAW). Lab Chip 9(23):3354–3359

    Google Scholar 

  27. Chen Y, Wu M, Ren L, Liu J, Whitley PH, Wang L, Huang TJ (2016) High-throughput acoustic separation of platelets from whole blood. Lab Chip 16(18):3466–3472

    Google Scholar 

  28. Han K-H, Bruno Frazier A (2004) Continuous magnetophoretic separation of blood cells in microdevice format. J Appl Phys 96(10):5797–5802

    Google Scholar 

  29. Pamme N, Manz A (2004) On-chip free-flow magnetophoresis: continuous flow separation of magnetic particles and agglomerates. Anal Chem 76(24):7250–7256

    Google Scholar 

  30. Han K-H, Frazier AB (2005) Diamagnetic capture mode magnetophoretic microseparator for blood cells. J Microelectromech Syst 14(6):1422–1431

    Google Scholar 

  31. Han K-H, Frazier AB (2005) A microfluidic system for continuous magnetophoretic separation of suspended cells using their native magnetic properties. Proc Nanotechol 1:187–190

    Google Scholar 

  32. Tzirtzilakis E (2005) A mathematical model for blood flow in magnetic field. Phys Fluids 17(7):077103

    MathSciNet  MATH  Google Scholar 

  33. Furlani EP (2007) Magnetophoretic separation of blood cells at the microscale. J Phys D Appl Phys 40(5):1313

    Google Scholar 

  34. Adams JD, Kim U, Soh HT (2008) Multitarget magnetic activated cell sorter. Proc Natl Acad Sci 105(47):18165–18170

    Google Scholar 

  35. Gijs MA, Lacharme F, Lehmann U (2010) Microfluidic applications of magnetic particles for biological analysis and catalysis. Chem Rev 110(3):1518–1563

    Google Scholar 

  36. Seo H-K, Kim H-O, Kim Y-J (2010) Hydrodynamics and magnetophoresis based hybrid blood cell sorter. In: 10th IEEE international conference on nanotechnology. IEEE, pp 911–914

  37. Seo H-K, Kim Y-H, Kim H-O, Kim Y-J (2010) Hybrid cell sorters for on-chip cell separation by hydrodynamics and magnetophoresis. J Micromech Microeng 20(9):095019

    Google Scholar 

  38. Zhu T, Marrero F, Mao L (2010) Continuous separation of non-magnetic particles inside ferrofluids. Microfluid Nanofluid 9(4–5):1003–1009

    Google Scholar 

  39. Baek MK, Choi HS, Lee KS, Park IH (2011) Numerical analysis for magnetophoretic separation of blood cells in fluid and magnetic field. IEEE Trans Appl Supercond 22(3):4401604

    Google Scholar 

  40. Forbes TP, Forry SP (2012) Microfluidic magnetophoretic separations of immunomagnetically labeled rare mammalian cells. Lab Chip 12(8):1471–1479

    Google Scholar 

  41. Mizuno M, Yamada M, Mitamura R, Ike K, Toyama K, Seki M (2013) Magnetophoresis-integrated hydrodynamic filtration system for size-and surface marker-based two-dimensional cell sorting. Anal Chem 85(16):7666–7673

    Google Scholar 

  42. Nam J, Huang H, Lim H, Lim C, Shin S (2013) Magnetic separation of malaria-infected red blood cells in various developmental stages. Anal Chem 85(15):7316–7323

    Google Scholar 

  43. Zhu T, Cheng R, Liu Y, He J, Mao L (2014) Combining positive and negative magnetophoreses to separate particles of different magnetic properties. Microfluid Nanofluid 17(6):973–982

    Google Scholar 

  44. Fateen S-EK, Magdy M (2015) Design. Three dimensional simulation of negative-magnetophoretic filtration of non-magnetic nanoparticles. Chem Eng Res Des 95:69–78

    Google Scholar 

  45. Hejazian M, Li W, Nguyen N-T (2015) Lab on a chip for continuous-flow magnetic cell separation. Lab Chip 15(4):959–970

    Google Scholar 

  46. Zhou Y, Kumar DT, Lu X, Kale A, DuBose J, Song Y, Wang J, Li D, Xuan X (2015) Simultaneous diamagnetic and magnetic particle trapping in ferrofluid microflows via a single permanent magnet. Biomicrofluidics 9(4):044102

    Google Scholar 

  47. Hejazian M, Nguyen N-T (2016) Magnetofluidic concentration and separation of non-magnetic particles using two magnet arrays. Biomicrofluidics 10(4):044103

    Google Scholar 

  48. Kim MJ, Lee DJ, Youn JR, Song YS (2016) Two step label free particle separation in a microfluidic system using elasto-inertial focusing and magnetophoresis. RSC Adv 6(38):32090–32097

    Google Scholar 

  49. Zhang J, Yan S, Yuan D, Zhao Q, Tan SH, Nguyen N-T, Li W (2016) A novel viscoelastic-based ferrofluid for continuous sheathless microfluidic separation of nonmagnetic microparticles. Lab Chip 16(20):3947–3956

    Google Scholar 

  50. Zhou Y, Xuan X (2016) Diamagnetic particle separation by shape in ferrofluids. Appl Phys Lett 109(10):102405

    Google Scholar 

  51. Chen Q, Li D, Lin J, Wang M, Xuan X (2017) Simultaneous separation and washing of nonmagnetic particles in an inertial ferrofluid/water coflow. Anal Chem 89(12):6915–6920

    Google Scholar 

  52. Tarn MD, Pamme N (2017) On-chip magnetic particle-based immunoassays using multilaminar flow for clinical diagnostics. Microchip diagnostics. Springer, New York, pp 69–83

    Google Scholar 

  53. Zhao W, Cheng R, Jenkins BD, Zhu T, Okonkwo NE, Jones CE, Davis MB, Kavuri SK, Hao Z, Schroeder C (2017) Label-free ferrohydrodynamic cell separation of circulating tumor cells. Lab Chip 17(18):3097–3111

    Google Scholar 

  54. Zhao W, Cheng R, Lim SH, Miller JR, Zhang W, Tang W, Xie J, Mao L (2017) Biocompatible and label-free separation of cancer cells from cell culture lines from white blood cells in ferrofluids. Lab Chip 17(13):2243–2255

    Google Scholar 

  55. Alnaimat F, Dagher S, Mathew B, Hilal-Alnqbi A, Khashan S (2018) Microfluidics based magnetophoresis: a review. Chem Rec 18(11):1596–1612

    Google Scholar 

  56. Cardoso VF, Miranda D, Botelho G, Minas G, Lanceros-Méndez S (2018) Highly effective clean-up of magnetic nanoparticles using microfluidic technology. Sens. Chem. Actuat. B 255:2384–2391

    Google Scholar 

  57. Munaz A, Shiddiky MJ, Nguyen N-T (2018) Recent advances and current challenges in magnetophoresis based micro magnetofluidics. Biomicrofluidics 12(3):031501

    Google Scholar 

  58. Munaz A, Shiddiky MJ, Nguyen N-T (2018) Chemical AB. Magnetophoretic separation of diamagnetic particles through parallel ferrofluid streams. Sens Chem Actuat B 275:459–469

    Google Scholar 

  59. Oh S, Jung SH, Seo H, Min M-K, Kim B, Hahn YK, Kang JH, Choi S (2018) Magnetic activated cell sorting (MACS) pipette tip for immunomagnetic bacteria separation. Sens Chem Actuat B 272:324–330

    Google Scholar 

  60. Wu J, Cui Y, Xuan S, Gong X (2018) Nanofluidics. 3D-printed microfluidic manipulation device integrated with magnetic array. Microfluidics and Nanofluidics 22(9):103

    Google Scholar 

  61. Lin S, Zhi X, Chen D, Xia F, Shen Y, Niu J, Huang S, Song J, Miao J, Cui D (2019) A flyover style microfluidic chip for highly purified magnetic cell separation. Biosens Bioelectron 129:175–181

    Google Scholar 

  62. Watarai H, Namba M (2002) Capillary magnetophoresis of human blood cells and their magnetophoretic trapping in a flow system. J Chromatogr A 961(1):3–8

    Google Scholar 

  63. Zborowski M, Ostera GR, Moore LR, Milliron S, Chalmers JJ, Schechter AN (2003) Red blood cell magnetophoresis. Biophys J 84(4):2638–2645

    Google Scholar 

  64. Vanderlinde J (2006) Classical electromagnetic theory. Springer, New York, p 145

    MATH  Google Scholar 

  65. Cheng R, Zhu T, Mao L (2014) Three-dimensional and analytical modeling of microfluidic particle transport in magnetic fluids. Microfluid Nanofluid 16(6):1143–1154

    Google Scholar 

  66. He Y, Luo L, Huang S (2019) Magnetic manipulation on the unlabeled nonmagnetic particles. Int J Mod Phys B 33(07):1950047

    Google Scholar 

  67. Bayareh M, Nazemi Ashani M, Usefian A (2020) Active and passive micromixers: a comprehensive review. Chem Eng Process Process Intensif 147:107771. https://doi.org/10.1016/j.cep.2019.107771

    Article  Google Scholar 

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  1. Department of Mechanical Engineering, Shahrekord University, Shahrekord, Iran

    Afshin Shiriny & Morteza Bayareh

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  1. Afshin Shiriny
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  2. Morteza Bayareh
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Correspondence to Morteza Bayareh.

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Shiriny, A., Bayareh, M. On magnetophoretic separation of blood cells using Halbach array of magnets. Meccanica 55, 1903–1916 (2020). https://doi.org/10.1007/s11012-020-01225-y

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